Voigt W H, Metzger K G
Arzneimittelforschung. 1979;29(12a):1939-41.
Investigations by electron microscopy on thin sections and negative-stained preparations were carried out to show the effect of 6-[(R)-2-[3-methylsulfonyl-2-oxo-imidazolidine-1-carboxamido]-2-phenyl-acetamido]-penicillanic acid sodium salt (mezlocillin, Baypen) on the ultrastructures of gramnegative bacteria (Escherichia coli C 165). Distinct alterations of the bacteria were to be seen depending on concentration and cultivation time. The outer wall of the bacterial cell became irregular. Locally it detached from the plasma membrane. The normal density of the cytoplasmic structures decreased quickly. Owing to their inability to form septa the bacteria grew as extended filaments. These mezlocillin-treated cells could form filaments of up to 100 times the length of normal untreated bacteria. Those bacterial filaments, however, were unable to form colonies as was shown by survival curves carried out in parallel. The filaments, therefore, were irreversibly damaged bacterial forms.
通过电子显微镜对超薄切片和负染制剂进行研究，以显示6-[(R)-2-[3-甲基磺酰基-2-氧代-咪唑烷-1-甲酰胺基]-2-苯基-乙酰胺基]-青霉烷酸钠盐（美洛西林，拜朋）对革兰氏阴性菌（大肠杆菌C 165）超微结构的影响。根据浓度和培养时间的不同，可以看到细菌有明显的变化。细菌细胞的外壁变得不规则。局部区域它与质膜分离。细胞质结构的正常密度迅速降低。由于无法形成隔膜，细菌长成了细长的丝状体。这些经美洛西林处理的细胞可以形成长达未处理正常细菌长度100倍的丝状体。然而，如平行进行的存活曲线所示，这些细菌丝状体无法形成菌落。因此，这些丝状体是不可逆损伤的细菌形态。
