Pardesi Bikiran, Roberton Anthony M, Wollmuth Emily M, Angert Esther R, Rosendale Douglas I, White Lindsey W, Clements Kendall D
School of Biological Sciences, University of Auckland, Auckland, New Zealand.
Department of Microbiology, Cornell University, Ithaca, NY, 14853, USA.
Int J Syst Evol Microbiol. 2024 Dec;74(12). doi: 10.1099/ijsem.0.006615.
A Gram-stain-negative, non-spore-forming, rod-shaped, obligately anaerobic bacterium, designated strain BP47G, was isolated from the hindgut of a silver drummer () fish collected from the Hauraki Gulf, New Zealand. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that it belonged to the family in the phylum . The gene sequence of BP47G was most similar to with 95.23% sequence identity. Isolate BP47G grew on agar medium containing mannitol and fish gut fluid as the sole carbon sources. Clear colonies of ~1 mm diameter grew within a week at 20-28 °C (optimum 28 °C) and pH 7.1-8.5 (optimum 8.5). BP47G tolerated the addition to the medium of up to 1% NaCl. Formate and butyrate were the major fermentation products. The major cellular fatty acids were C, C, iso-C, C and C 7. Genomic analyses comparing BP47G with its closest relatives indicated low genomic relatedness based on the average nucleotide identity, average amino acid identity, percentage of conserved protein and DNA-DNA hybridization. Supported by the phenotypic and taxonomic characteristics observed in this study, a novel genus and species gen. nov., sp. nov. is proposed for isolate BP47G (=ICMP 24688=JCM 35770).
从新西兰豪拉基湾采集的一条银鼓鱼（）的后肠中分离出一株革兰氏染色阴性、不形成芽孢、杆状、专性厌氧细菌，命名为BP47G菌株。基于该分离株16S rRNA基因序列的系统发育分析表明，它属于门中的科。BP47G的基因序列与序列相似性最高，为95.23%。分离株BP47G在含有甘露醇和鱼肠液作为唯一碳源的琼脂培养基上生长。在20-28°C（最适温度28°C）和pH 7.1-8.5（最适pH 8.5）条件下，一周内可长出直径约1 mm的透明菌落。BP47G能耐受培养基中添加高达1%的NaCl。甲酸和丁酸是主要发酵产物。主要细胞脂肪酸为C、C、异-C、C和C 7。对BP47G与其最接近的亲缘种进行的基因组分析表明，基于平均核苷酸同一性、平均氨基酸同一性、保守蛋白百分比和DNA-DNA杂交，其基因组相关性较低。根据本研究中观察到的表型和分类学特征，建议将分离株BP47G（=ICMP 24688=JCM 35770）定为一个新属新种，属名新属，种名新种。
