School of Biological Sciences, University of Auckland, Auckland, New Zealand.
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005374.
A Gram-stain-positive, non-spore-forming, rod-shaped, obligately anaerobic bacterium, designated strain BP52G, was isolated from the hindgut of a Silver Drummer () fish collected from the Hauraki Gulf, New Zealand. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belonged to the family in the phylum Firmicutes and was most closely related to with 93.3 % sequence identity. Isolate BP52G grew on agar medium containing mannitol as the sole carbon source. White, opaque and shiny colonies of the isolate measuring approximately 1 mm diameter grew within a week at 20-28 °C (optimum, 24 °C) and pH 6.9-8.5 (optimum, pH 7.8). BP52G tolerated the addition of up to 1 % NaCl to the medium. Formate and acetate were the major fermentation products. The major cellular fatty acids were C, C and C. The genome sequence of the isolate was determined. Its G+C content was 30.7 mol%, and the 72.65 % average nucleotide identity of the BP52G genome to its closest neighbour with a completely sequenced genome ( JCM 1298) indicated low genomic relatedness. Based on the phenotypic and taxonomic characteristics observed in this study, a novel genus and species gen. nov., sp. nov. is proposed for isolate BP52G (=NZRM 4757=JCM 34692).
一株革兰氏阳性、非孢子形成、杆状、严格厌氧细菌，命名为 BP52G 菌株，从新西兰豪拉基湾采集的银鼓鱼的后肠中分离得到。基于 16S rRNA 基因测序的系统发育分析表明，该分离物属于厚壁菌门Firmicutes 中的 科，与 具有最高的序列同一性（93.3%）。分离物 BP52G 在含有甘露醇作为唯一碳源的琼脂培养基上生长。该分离物的白色、不透明和有光泽的菌落大约 1mm 直径，在 20-28°C（最佳温度为 24°C）和 pH 6.9-8.5（最佳 pH 为 7.8）下培养一周内即可生长。BP52G 可以耐受培养基中添加高达 1%的 NaCl。其主要发酵产物为甲酸盐和乙酸盐。主要细胞脂肪酸为 C、C 和 C。该分离物的基因组序列已被确定。其 G+C 含量为 30.7mol%，与具有完全测序基因组的最接近的近缘种（JCM 1298）的 BP52G 基因组的 72.65%平均核苷酸同一性表明其基因组相关性较低。根据本研究中观察到的表型和分类特征，建议将分离物 BP52G（=NZRM 4757=JCM 34692）命名为一个新属和新种， 属，种。nov.。
