Boutom Sarah M, Silva Teresa P, Palecek Sean P, Shusta Eric V, Fernandes Tiago G, Ashton Randolph S
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Department of Bioengineering and IBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; Associate Laboratory i4HB-Institute for Health and Bioeconomy, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.
Cell Rep. 2024 Dec 24;43(12):115068. doi: 10.1016/j.celrep.2024.115068. Epub 2024 Dec 16.
In recent years, neural organoids derived from human pluripotent stem cells (hPSCs) have offered a transformative pre-clinical platform for understanding central nervous system (CNS) development, disease, drug effects, and toxicology. CNS vasculature plays an important role in all these scenarios; however, most published studies describe CNS organoids that lack a functional vasculature or demonstrate rudimentary incorporation of endothelial cells or blood vessel networks. Here, we review the existing knowledge of vascularization during the development of different CNS regions, including the brain, spinal cord, and retina, and compare it to vascularized CNS organoid models. We highlight several areas of contrast where further bioengineering innovation is needed and discuss potential applications of vascularized neural organoids in modeling human CNS development, physiology, and disease.
近年来，源自人类多能干细胞（hPSC）的神经类器官为理解中枢神经系统（CNS）发育、疾病、药物作用及毒理学提供了一个变革性的临床前平台。CNS脉管系统在所有这些情况中都发挥着重要作用；然而，大多数已发表的研究描述的是缺乏功能性脉管系统或仅显示内皮细胞或血管网络初步整合的CNS类器官。在这里，我们回顾了不同CNS区域（包括脑、脊髓和视网膜）发育过程中血管化的现有知识，并将其与血管化的CNS类器官模型进行比较。我们强调了几个需要进一步生物工程创新的对比领域，并讨论了血管化神经类器官在模拟人类CNS发育、生理学和疾病方面的潜在应用。
