单细胞RNA测序揭示了驴精子发生和成熟过程中的动态转录谱。
Single-cell RNA sequencing unveils dynamic transcriptional profiles during the process of donkey spermatogenesis and maturation.
作者信息
Jin Yadan, Zhang Fangdi, Ma Ruixue, Xing Jingya, Wang Min, Sun Yujiang, Zhang Guoliang
机构信息
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
出版信息
Genomics. 2025 Jan;117(1):110974. doi: 10.1016/j.ygeno.2024.110974. Epub 2024 Dec 16.
INTRODUCTION
With the increasing demand for donkey production, there has been a growing focus on the breeding of donkeys. However, our current understanding of the mechanisms underlying spermatogenesis and maturation in donkeys during reproduction remains limited.
OBJECTIVES
This study is to provide a comprehensive single-cell landscape analysis of spermatogenesis and maturation in donkeys.
METHODS
In this study, we employed single-cell RNA sequencing to investigate cell composition, gene expression patterns, and regulatory roles during spermatogenesis and maturation in donkeys.
RESULTS
The expression patterns of CDK1, CETN3, and UBE2J1 were found to be indicative of specific germ cells during donkey spermatogenesis. Additionally, the DEFB121, ELSPBP1, and NPC2 genes were specifically identified in the principal cells of the donkey epididymis.
CONCLUSIONS
We performed single-cell RNA sequencing to analyze the cellular composition and spatial distribution of donkey testis and epididymis, thereby generating comprehensive transcriptional atlases at the single-cell resolution.
引言
随着对驴产品需求的增加，对驴的繁殖越来越受到关注。然而，我们目前对驴在繁殖过程中精子发生和成熟的潜在机制的了解仍然有限。
目的
本研究旨在对驴的精子发生和成熟进行全面的单细胞图谱分析。
方法
在本研究中，我们采用单细胞RNA测序来研究驴精子发生和成熟过程中的细胞组成、基因表达模式及调控作用。
结果
发现CDK1、CETN3和UBE2J1的表达模式可指示驴精子发生过程中的特定生殖细胞。此外，在驴附睾的主细胞中特异性鉴定出DEFB121、ELSPBP1和NPC2基因。
结论
我们进行了单细胞RNA测序以分析驴睾丸和附睾的细胞组成及空间分布，从而在单细胞分辨率下生成了全面的转录图谱。
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