Otsuki Kouharu, Hosoya Chihiro, Takamiya Riko, Kimura Mayu, Kikuchi Takashi, Huang Li, Chen Chin-Ho, Li Wei
Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba, 274-8510, Japan.
Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba, 274-8510, Japan.
Phytochemistry. 2025 Apr;232:114366. doi: 10.1016/j.phytochem.2024.114366. Epub 2024 Dec 16.
The plants of the genus Daphne (Thymelaeaceae) are known to be sources of anti-HIV diterpenoids. Therefore, the present study focused on investigating the anti-HIV diterpenoids in Daphne pseudomezereum, for which the isolation of diterpenoids has not been previously reported. The results showed that three previously undescribed daphnane diterpenoids, onishibarins A-C (1-3), along with seven known compounds (4-10) were isolated from the fruits of Daphne pseudomezereum. Their structures were established by comprehensive analysis of physicochemical and spectroscopic data. Evaluation of the anti-HIV activity and cytotoxicity of the isolated compounds showed that compounds 1, 9, and 10 exhibited potent anti-HIV activity at EC = 1.26, 0.84, and 0.78 nM, respectively, with cytotoxicity at IC > 5 μM.
瑞香属（瑞香科）植物是抗HIV二萜类化合物的来源。因此，本研究着重于调查黄瑞香中的抗HIV二萜类化合物，此前尚未有关于黄瑞香二萜类化合物分离的报道。结果表明，从黄瑞香果实中分离出了三种此前未描述过的瑞香烷二萜类化合物，鬼吹箫素A - C（1 - 3），以及七种已知化合物（4 - 10）。通过对物理化学和光谱数据的综合分析确定了它们的结构。对分离得到的化合物的抗HIV活性和细胞毒性评估表明，化合物1、9和10分别在EC = 1.26、0.84和0.78 nM时表现出强效抗HIV活性，细胞毒性在IC > 5 μM。
