Masroor Azin, Gholipour Abolfazl, Shahini Shams Abadi Milad, Mousavi Mohammad, Hadi Alijanvand Moluk, Zamanzad Behnam
Department of Medical Microbiology and Immunology, Faculty of Medicine, Shahrekord Medical University, Shahrekord, Iran.
AMB Express. 2024 Dec 18;14(1):138. doi: 10.1186/s13568-024-01785-2.
Rheumatoid arthritis (RA) is an autoimmune disorder with synovial inflammation of joints and extra articular manifestations. The results of recent researches consider the relationship between microbiota and the immune system as a double-edged sword. Considering that the relationship between the composition of intestinal microbiota and the immunological and clinical status of the body has been confirmed, it is very important to investigate the effect of each genus and species of bacteria on the state of the immune system. The current study was determined using 16S rRNA gene sequencing to explore the 4 selected gut microbiota from 25 people suffering from rheumatism (RA group) with a time interval of at least 3 years from the onset of the disease and 25 Healthy people by real time PCR. Gut dysbiosis in patients with rheumatoid arthritis (RA) is identified alongside key serological and clinical markers such as C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), immunofluorescence (IF), anti-cyclic citrullinated peptide (Anti-CCP), white blood cell count (WBC), mean corpuscular volume (MCV), aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), Platelet count (PLT), thyroid-stimulating hormone (TSH), creatinine (Cr), and hemoglobin (Hb). Additionally, data from individuals with incomplete or unverified records were excluded from the study to ensure accuracy and reliability. Bacteroides fragilis, Roseburia faecis and Fusobacterium nucleatum genera showed a much lower median in Rheumatoid arthritis patients in comparison with healthy people (P > 0.001, p = 0.002, P < 0.001 respectively). While the difference in the median of E. coli genera was not significant in the two studied groups (p = 0.31). In such a way that the change in the Gut normal flora homeostasis and the reduction of beneficial bacteria such as Bacteroides fragilis, Roseburia faecis, Fusobacterium nucleatum genera, may stimulate the immune system to initiate autoimmunity.
类风湿性关节炎（RA）是一种自身免疫性疾病，伴有关节滑膜炎症和关节外表现。最近的研究结果认为微生物群与免疫系统之间的关系是一把双刃剑。鉴于肠道微生物群的组成与身体的免疫和临床状态之间的关系已得到证实，研究每种细菌属和种对免疫系统状态的影响非常重要。本研究采用16S rRNA基因测序法，通过实时PCR从25名患有风湿病的患者（RA组）中选取4种肠道微生物群进行研究，这些患者自疾病发作起至少有3年时间间隔，同时选取25名健康人作为对照。类风湿性关节炎（RA）患者的肠道菌群失调与关键的血清学和临床标志物一同被识别出来，这些标志物包括C反应蛋白（CRP）、红细胞沉降率（ESR）、免疫荧光（IF）、抗环瓜氨酸肽（Anti-CCP）、白细胞计数（WBC）、平均红细胞体积（MCV）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、血尿素氮（BUN）、血小板计数（PLT）、促甲状腺激素（TSH）、肌酐（Cr）和血红蛋白（Hb）。此外，来自记录不完整或未经核实的个体的数据被排除在研究之外，以确保准确性和可靠性。与健康人相比，脆弱拟杆菌、粪便罗氏菌和具核梭杆菌属在类风湿性关节炎患者中的中位数要低得多（分别为P＞0.001、p = 0.002、P＜0.001）。而大肠杆菌属在两个研究组中的中位数差异不显著（p = 0.31）。由此可见，肠道正常菌群稳态的改变以及脆弱拟杆菌、粪便罗氏菌、具核梭杆菌属等有益菌数量的减少，可能会刺激免疫系统引发自身免疫。
