Mirsalami Seyed Mehrdad, Mirsalami Mahsa
Department of Chemical Engineering, Faculty of Engineering, Islamic Azad University Central Tehran Branch, Tehran, Iran.
Faculty of Engineering and Technical Sciences, Qazvin Islamic Azad University, Qazvin, Iran.
AMB Express. 2024 Dec 18;14(1):137. doi: 10.1186/s13568-024-01792-3.
Anaerobic bacteria, such as Lactobacillus plantarum (LP), are known to play a significant role in maintaining gut health and protecting against enteric pathogens in animals. The present study aimed to develop a safe, affordable, and eco-friendly method for producing LP-based probiotics and evaluate their efficacy in mitigating Salmonella-induced diarrhea in broiler chickens. The study employed three different culture media (MRS, TSB, and Baird Parker) to grow LP, which was then dried using a spray-drying technique to produce a stable probiotic formulation. When administered to broiler chickens, the LP probiotic derived from the MRS medium significantly improved body weight gain (4.147-fold increase over 4 weeks) compared to the other two culture conditions. Importantly, the LP probiotic treatment could substantially reduce the diarrhea index in broilers, with up to an 86.45% improvement in Salmonella-induced enteric infections. The beneficial effects were attributed to the ability of LP to modulate the gut microbiome, enhance the integrity of the intestinal mucosa, and mitigate the pathogenic effects of Salmonella. These findings demonstrate the potential of anaerobic Lactobacillus plantarum as a safe and effective probiotic intervention for controlling enteric diseases and improving production outcomes in poultry farming. The developed method provides a sustainable approach to harness the beneficial properties of this anaerobic bacterium for animal health and welfare.
厌氧细菌，如植物乳杆菌（LP），已知在维持动物肠道健康和抵御肠道病原体方面发挥着重要作用。本研究旨在开发一种安全、经济且环保的方法来生产基于LP的益生菌，并评估其减轻肉鸡沙门氏菌诱导腹泻的功效。该研究采用三种不同的培养基（MRS、TSB和贝尔德·帕克培养基）来培养LP，然后使用喷雾干燥技术将其干燥，以生产稳定的益生菌制剂。当给肉鸡施用时，源自MRS培养基的LP益生菌与其他两种培养条件相比，显著提高了体重增加（4周内增加了4.147倍）。重要的是，LP益生菌处理可大幅降低肉鸡的腹泻指数，沙门氏菌诱导的肠道感染改善高达86.45%。这些有益效果归因于LP调节肠道微生物群、增强肠黏膜完整性以及减轻沙门氏菌致病作用的能力。这些发现表明，厌氧植物乳杆菌作为一种安全有效的益生菌干预措施，在控制家禽养殖中的肠道疾病和改善生产成果方面具有潜力。所开发的方法为利用这种厌氧细菌的有益特性促进动物健康和福利提供了一种可持续的途径。
