Dos Santos Jana Kelly, do Nascimento Sergio Alves, Silva Karla Patrícia Chaves, Medeiros Elizabeth Sampaio, de Carvalho Maia Rita Cássia, Pinheiro Junior José Wilton
Federal Institute of Education, Science and Technology of Alagoas (IFAL), R. 17 de Agosto, s/n - Zona Rural, Satuba, Alagoas, 57120-000, Brazil.
Federal Rural University of Pernambuco (UFRPE), R. Dom Manuel de Medeiros, s/n - Dois Irmãos, Recife, Pernambuco, 52171-900, Brazil.
Trop Anim Health Prod. 2024 Dec 19;57(1):2. doi: 10.1007/s11250-024-04255-w.
The objective of this study was to conduct a serological investigation of Schmallenberg virus (SBV) infection in cattle raised in the State of Alagoas. A total of 460 serum samples from the Agricultural Defense Agency of Alagoas (ADEAL) serum bank were analyzed. These samples were collected from unvaccinated cattle on 100 properties across 99 municipalities in Alagoas, representing the three mesoregions: Agreste, Leste, and Sertão. Serological diagnosis was performed using commercial indirect Enzyme-Linked Immunosorbent Assay (ELISA) kit. The results showed that 0.87% (4 out of 460) of the samples yielded questionable results in the indirect ELISA (with calculated percentages > 50.00% and ≤ 60.00%). Upon further testing with competitive ELISA, only one sample tested positive. These findings underscore the need to strengthen regional surveillance and control strategies for emerging pathogens. Additionally, further studies, including viral isolation from both ruminants and vectors, are crucial to confirm the circulation of this pathogen. Such comprehensive investigations are essential for improving our understanding of SBV dynamics and for developing effective control measures.
本研究的目的是对阿拉戈斯州饲养的牛进行施马伦贝格病毒（SBV）感染的血清学调查。对阿拉戈斯州农业防御局（ADEAL）血清库的460份血清样本进行了分析。这些样本采自阿拉戈斯州99个市100个养殖场的未接种疫苗的牛，代表了三个中区域：滨海平原、东部和半干旱地区。使用商业间接酶联免疫吸附测定（ELISA）试剂盒进行血清学诊断。结果显示，0.87%（460份样本中的4份）的样本在间接ELISA中产生了可疑结果（计算百分比>50.00%且≤60.00%）。在用竞争ELISA进一步检测后，只有一份样本检测呈阳性。这些发现强调了加强对新兴病原体的区域监测和控制策略的必要性。此外，进一步的研究，包括从反刍动物和媒介中分离病毒，对于确认这种病原体的传播至关重要。此类全面调查对于增进我们对SBV动态的了解以及制定有效的控制措施至关重要。
