Wang Zhiqiang, Qi Yunjia, Xiao Nan, She Liu, Zhang Yunchuang, Lu Junzhi, Jiang Qinyang, Luo Chan
Guangxi Academy of Medical Sciences and the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, China.
Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, 75 Xiuling Road, Nanning, 530005, China.
BMC Genomics. 2024 Dec 18;25(1):1195. doi: 10.1186/s12864-024-11094-4.
The establishment and maintenance of spermatogenesis is a complex process involving a vast of regulatory pathways. There is growing evidence revealing that long noncoding RNAs (lncRNA) play important roles in regulating testicular development and spermatogenesis in a stage-specific way. However, our understanding of how lncRNA regulates testicular development and spermatogenesis in black goats is quite limited. In the current study, we screened the transcriptomes (lncRNA and mRNA) of testicular from Guangxi black goats before puberty (3 days old, D3; 30 days old, D30), puberty (90 days old, D90) and postpuberty (180 days old, D180), in order to identify the lncRNA interaction with mRNAs contributes to goat spermatogenesis. The RNA-sequencing (RNA-seq) analysis showed that there were 1211, 12,180, 834 differential lncRNAs and 1196, 8838,269 differential mRNAs at the ages of D30 vs. D3, D90 vs. D30, and D180 vs. D90. The lncRNAs showed the most significantly changes from D30 to D90, which indicated that D90 was a key node of lncRNAs participated in the regulation of testicular development and spermatogenesis in black goat. According to functional enrichment analysis of GO and KEGG, we found that differentially expressed lncRNAs (DE lncRNAs) and their target genes regulated spermatogenesis through signal pathways including MAPK, Ras, and PI3K-Akt. Using cis- and trans-acting, 39 DE lncRNAs-targeted genes were found to be enriched for male reproduction. Of these, LOC108635509, which specific expressed in testis and upregulated the expression levels at D90, was found participated in the regulation of testicular development through promoting the proliferation of Sertoli cells (SCs). Overall, this study provides new insight into the regulatory mechanisms that support spermatogenesis and testicular development in black goats.
精子发生的建立和维持是一个复杂的过程，涉及大量的调控途径。越来越多的证据表明，长链非编码RNA（lncRNA）以阶段特异性的方式在调节睾丸发育和精子发生中发挥重要作用。然而，我们对lncRNA如何调节黑山羊睾丸发育和精子发生的了解相当有限。在本研究中，我们筛选了广西黑山羊青春期前（3日龄，D3；30日龄，D30）、青春期（90日龄，D90）和青春期后（180日龄，D180）睾丸的转录组（lncRNA和mRNA），以鉴定与mRNA相互作用的lncRNA对山羊精子发生的作用。RNA测序（RNA-seq）分析表明，在D30与D3、D90与D30、D180与D90时，分别有1211、12180、834个差异lncRNA和1196、8838、269个差异mRNA。lncRNA在D30到D90之间变化最为显著，这表明D90是lncRNA参与调节黑山羊睾丸发育和精子发生的关键节点。根据GO和KEGG的功能富集分析，我们发现差异表达的lncRNA（DE lncRNA）及其靶基因通过包括MAPK、Ras和PI3K-Akt在内的信号通路调节精子发生。利用顺式和反式作用，发现39个DE lncRNA靶向基因在雄性生殖方面富集。其中，在睾丸中特异性表达并在D90时上调表达水平的LOC108635509被发现通过促进支持细胞（SCs）的增殖参与睾丸发育的调节。总体而言，本研究为支持黑山羊精子发生和睾丸发育的调控机制提供了新的见解。
