Yang Jia, Li Li, Xu Fuqiang, Jia Fan
Shenzhen Key Laboratory of Viral Vectors for Biomedicine, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Key Laboratory of Quality Control Technology for Virus-Based Therapeutics, the Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Vet Res. 2024 Dec 18;55(1):167. doi: 10.1186/s13567-024-01430-7.
Feline herpesvirus type 1 (FHV-1), a member of the Herpesviridae family, is one of the most important pathogens that causes upper respiratory tract disease in felines. Following infection, FHV-1 can spread retrogradely to the trigeminal ganglia, establishing a life-long latency. Although vaccines are available for routine feline vaccination, FHV-1 is still an agent that poses a serious threat to feline health. There are currently no specific drugs for the treatment of FHV-1. To facilitate the screening of antiviral drugs, we constructed a reporter FHV-1 virus, which expresses a secreted Gaussia luciferase (GLuc) and a bright green fluorescent protein, mNeonGreen. The reporter virus shows slower growth than does the wild-type FHV-1. The expression of the two reporter genes, Gluc and mNeonGreen, was consistent with viral propagation and remained stable during continuous passage in CRFK cells, even after twenty rounds. In addition, the known inhibitor ganciclovir was used to confirm the characteristics of the reporter virus for drug screening. We found that the reporter FHV-1 is suitable for antiviral screening assays. Overall, our work provides a useful tool for screening drugs to combat FHV-1.
猫疱疹病毒1型（FHV-1）是疱疹病毒科的成员之一，是引起猫上呼吸道疾病的最重要病原体之一。感染后，FHV-1可逆行扩散至三叉神经节，建立终身潜伏感染。尽管有疫苗可用于猫的常规疫苗接种，但FHV-1仍然是对猫健康构成严重威胁的病原体。目前尚无治疗FHV-1的特效药物。为便于筛选抗病毒药物，我们构建了一种报告型FHV-1病毒，其表达分泌型高斯荧光素酶（GLuc）和亮绿色荧光蛋白mNeonGreen。该报告病毒的生长速度比野生型FHV-1慢。两个报告基因Gluc和mNeonGreen的表达与病毒增殖一致，并且在CRFK细胞中连续传代期间保持稳定，即使经过二十代也是如此。此外，使用已知的抑制剂更昔洛韦来确认报告病毒用于药物筛选的特性。我们发现报告型FHV-1适用于抗病毒筛选试验。总体而言，我们的工作为筛选抗FHV-1的药物提供了有用的工具。
