Fook Karina Donato, Araújo Maria José Abigail Mendes, Muniz Alessandra Costa de Sales, Carvalho Mônika Machado de, Morais Ana Cléa Cutrim Diniz de, Araújo Deborah Rocha de, Guimarães Sulayne Janayna Araújo, Souza Camila Penha Abreu, Barbosa Carla Déa Trindade, Bertolaccini Maria Fernanda Lima, Belfort Ilka Kassandra Pereira, Lopes Fernanda Ferreira, Monteiro Sally Cristina Moutinho
Universidade Federal do Maranhão, Programa de Pós-Graduação em Saúde do Adulto, São Luís, Maranhão, Brazil.
Universidade Federal do Maranhão, Hospital Universitário, Laboratório de Análises Clínicas e Histocompatibilidade, São Luís, Maranhão, Brazil.
Rev Inst Med Trop Sao Paulo. 2024 Dec 6;66:e67. doi: 10.1590/S1678-9946202466067. eCollection 2024.
This study aims to investigate the possible association between neutrophil/lymphocyte and platelet/lymphocyte ratio in women with HIV, undergoing antiretroviral treatment, with HPV coinfection. This is a cross-sectional study with HIV positive women; their biological samples were collected for laboratory tests (complete blood count) and oncotic cytology for detection of HPV DNA, by PCR-Nested (PGMY and GP primers). Viral load and CD4 and CD8 T-cells counts were obtained from medical records. The data were analyzed, comparing the two groups: those with coinfection and those without it. From 82 HIV seropositive women, 50% exhibited HPV coinfection and 12.2% of coinfected patients had cervical cell alterations. Quantification of viral load, CD4 and CD8 T-cells count, CD4 / CD8 ratio and neutrophil/lymphocyte (NLR) and platelet/lymphocyte (PLR) ratio presented significant differences between groups (p<0.05). The predicting power of NLR and PLR in differentiating HIV/HPV coinfection which demonstrated differences between groups (AUC of 0.882 and 0.776 for NLR and PLR, respectively). There is a relation between the neutrophil/lymphocyte and platelet/lymphocyte ratio with HIV/HPV coinfection in women undergoing antiretroviral treatment, suggesting a state of greater and persistent systemic inflammation, reflecting as a biomarker for screening and monitoring these patients.
本研究旨在调查接受抗逆转录病毒治疗且合并人乳头瘤病毒（HPV）感染的HIV女性患者中，中性粒细胞/淋巴细胞比值和血小板/淋巴细胞比值之间可能存在的关联。这是一项针对HIV阳性女性的横断面研究；采集她们的生物样本进行实验室检测（全血细胞计数），并通过巢式PCR（PGMY和GP引物）进行渗透压细胞学检查以检测HPV DNA。病毒载量以及CD4和CD8 T细胞计数从病历中获取。对数据进行分析，比较两组：合并感染组和未合并感染组。在82名HIV血清阳性女性中，50%表现出HPV合并感染，12.2%的合并感染患者有宫颈细胞改变。病毒载量、CD4和CD8 T细胞计数、CD4/CD8比值以及中性粒细胞/淋巴细胞（NLR）和血小板/淋巴细胞（PLR）比值在两组之间存在显著差异（p<0.05）。NLR和PLR在区分HIV/HPV合并感染方面的预测能力显示出两组之间的差异（NLR和PLR的曲线下面积分别为0.882和0.776）。接受抗逆转录病毒治疗的女性中，中性粒细胞/淋巴细胞比值和血小板/淋巴细胞比值与HIV/HPV合并感染之间存在关联，这表明存在更严重且持续的全身炎症状态，可作为这些患者筛查和监测的生物标志物。
