Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Anzio Road, Observatory, 7925 Cape Town, South Africa.
BMC Infect Dis. 2014 Jan 31;14:51. doi: 10.1186/1471-2334-14-51.
Persistent high-risk (HR) human papillomavirus (HPV) infection and increased HR-HPV viral load are associated with the development of cancer. This study investigated the effect of human immunodeficiency virus (HIV) co-infection, HIV viral load and CD4 count on the HR-HPV viral load; and also investigated the predictors of cervical abnormalities.
Participants were 292 HIV-negative and 258 HIV-positive women. HR-HPV viral loads in cervical cells were determined by the real-time polymerase chain reaction.
HIV-positive women had a significantly higher viral load for combined alpha-9 HPV species compared to HIV-negative women (median 3.9 copies per cell compared to 0.63 copies per cell, P = 0.022). This was not observed for individual HPV types. HIV-positive women with CD4 counts >350/μl had significantly lower viral loads for alpha-7 HPV species (median 0.12 copies per cell) than HIV-positive women with CD4 ≤350/μl (median 1.52 copies per cell, P = 0.008), but low CD4 count was not significantly associated with increased viral load for other HPV species. High viral loads for alpha-6, alpha-7 and alpha-9 HPV species were significant predictors of abnormal cytology in women.
HIV co-infection significantly increased the combined alpha-9 HPV viral load in women but not viral loads for individual HPV types. High HR-HPV viral load was associated with cervical abnormal cytology.
持续性高危型（HR）人乳头瘤病毒（HPV）感染和 HR-HPV 病毒载量增加与癌症的发生有关。本研究调查了人类免疫缺陷病毒（HIV）合并感染、HIV 病毒载量和 CD4 计数对 HR-HPV 病毒载量的影响；并探讨了宫颈异常的预测因素。
参与者为 292 名 HIV 阴性和 258 名 HIV 阳性妇女。采用实时聚合酶链反应检测宫颈细胞中的 HR-HPV 病毒载量。
HIV 阳性妇女的联合α-9 HPV 种病毒载量明显高于 HIV 阴性妇女（中位数 3.9 拷贝/细胞比 0.63 拷贝/细胞，P=0.022）。但未观察到个别 HPV 类型存在这种情况。CD4 计数>350/μl 的 HIV 阳性妇女的α-7 HPV 种病毒载量明显低于 CD4 计数≤350/μl 的 HIV 阳性妇女（中位数 0.12 拷贝/细胞比 1.52 拷贝/细胞，P=0.008），但低 CD4 计数与其他 HPV 种病毒载量增加无显著相关性。α-6、α-7 和α-9 HPV 种的高病毒载量是妇女异常细胞学的显著预测因素。
HIV 合并感染显著增加了女性的联合α-9 HPV 病毒载量，但不增加个别 HPV 类型的病毒载量。高 HR-HPV 病毒载量与宫颈异常细胞学有关。
