Alves Marcia B, Nascimento Katyane S, Araújo Jéssica Mayara M, Firmo Wellyson C A, Mouchrek Filho Victor Elias, Sousa Dionney A DE, Guaraldi Ana Luíza M, Lima Neto Lídio G, Sabbadini Priscila S
Universidade CEUMA, Laboratório de Cências Biomédicas, Rua Josué Montello, 1, Renascença II, 65075-120 São Luís, MA, Brazil.
Universidade Federal do Maranhão, Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Rede BIONORTE, Av. dos Portugueses, 1966, Vila Bacanga, 65080-805 São Luís, MA, Brazil.
An Acad Bras Cienc. 2024 Dec 13;96(suppl 3):e20230368. doi: 10.1590/0001-3765202420230368. eCollection 2024.
The objective of this study was to investigate the chemical composition of Syzygium aromaticum essential oil (SAEO), both as its toxicity and biological activities on Corynebacterium diphtheriae. The essential oil (EO) was obtained by hydrodistillation and verified by GC-MS. The main chemical components were eugenol (48.79%), caryophyllene (44.29%) and α-humulene (4.87%). In vitro tests with human red blood cells, blood compatibility. SAEO showed high toxicity for Artemia salina and the bioassay on Tenebrio molitor larvae revealed an average survival rate of 80.00%. The antioxidant activity of SAEO was moderate (40.60%) using the phosphomolybdenum method. SAEO inhibited the growth of all C. diphtheriae strains tested at minimum inhibitory concentrations of 62.5-1000 µg mL-1 (strong to moderate). The minimum bactericidal concentration of SAEO was observed in two strains. Microorganisms cultivated in the presence of subinhibitory concentrations (subMIC) do not show morphological changes. The subMIC of SAEO inhibited in some strains, but in most cases, increased biofilm formation. In conclusion, the results of this study found that SAEO has an inhibitory effect against C. diphtheriae, representing an alternative antimicrobial therapy, although more in vivo biological studies, alone or in combination with occasional antimicrobials, should be carried out to better evaluate its effects.
本研究的目的是调查丁香精油（SAEO）的化学成分及其对白喉棒状杆菌的毒性和生物活性。通过水蒸馏法获得精油（EO），并通过气相色谱 - 质谱联用仪（GC - MS）进行验证。其主要化学成分是丁香酚（48.79%）、石竹烯（44.29%）和α-葎草烯（4.87%）。用人红细胞进行体外试验，检测血液相容性。SAEO对卤虫显示出高毒性，对黄粉虫幼虫的生物测定显示平均存活率为80.00%。采用磷钼酸法测定，SAEO的抗氧化活性为中等（40.60%）。SAEO在最低抑菌浓度为62.5 - 1000 µg mL-1（强至中等）时抑制所有测试的白喉棒状杆菌菌株的生长。在两个菌株中观察到了SAEO的最低杀菌浓度。在亚抑菌浓度（subMIC）存在下培养的微生物未显示出形态变化。SAEO的亚抑菌浓度在一些菌株中具有抑制作用，但在大多数情况下，会增加生物膜的形成。总之，本研究结果发现SAEO对白喉棒状杆菌具有抑制作用，代表了一种替代抗菌疗法，不过应该进行更多的体内生物学研究，单独或与其他抗菌药物联合使用，以更好地评估其效果。
