Fredriksen Simen, Neila-Ibáñez Carlos, Hennig-Pauka Isabel, Guan Xiaonan, Dunkelberger Jenelle, de Oliveira Isabela Fernandes, Ferrando Maria Laura, Correa-Fiz Florencia, Aragon Virginia, Boekhorst Jos, van Baarlen Peter, Wells Jerry M
Host-Microbe Interactomics Group, Animal Sciences Department, Wageningen University, Wageningen, Netherlands.
Unitat mixta dInvestigaci IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CRSA), Campus de laUniversitat Autnoma de Barcelona (UAB), Bellaterra, 08193, Catalonia, Spain.
Microb Genom. 2024 Dec;10(12). doi: 10.1099/mgen.0.001334.
is a Gram-positive opportunistic pathogen causing systemic disease in piglets around weaning age. The factors predisposing to disease are not known. We hypothesized that the tonsillar microbiota might influence disease risk via colonization resistance and/or co-infections. We conducted a cross-sectional case-control study within outbreak farms complemented by selective longitudinal sampling and comparison with control farms without disease occurrence. We found a small but significant difference in tonsil microbiota composition between case and control piglets (=45+45). Variants of putative commensal taxa, including , were reduced in abundance in case piglets compared to asymptomatic controls. Case piglets had higher relative abundances of , and uncultured and species. Piglets developing disease post-weaning had reduced alpha diversity pre-weaning. Despite case-control pairs receiving equal antimicrobial treatment, case piglets had a higher abundance of antimicrobial resistance genes conferring resistance to antimicrobial classes used to treat . This might be an adaption of disease-associated strains to frequent antimicrobial treatment.
是一种革兰氏阳性机会致病菌，可在断奶前后的仔猪中引起全身性疾病。导致疾病的易感因素尚不清楚。我们假设扁桃体微生物群可能通过定植抗性和/或共同感染影响疾病风险。我们在暴发猪场中进行了一项横断面病例对照研究，并辅以选择性纵向采样，并与无疾病发生的对照猪场进行比较。我们发现病例仔猪和对照仔猪的扁桃体微生物群组成存在微小但显著的差异（=45+45）。与无症状对照相比，病例仔猪中包括 在内的假定共生类群的变体丰度降低。病例仔猪中 、 和未培养的 及 物种的相对丰度较高。断奶后发病的仔猪断奶前的α多样性降低。尽管病例对照对接受了相同的抗菌治疗，但病例仔猪中赋予对用于治疗 的抗菌类别的抗性的抗菌抗性基因丰度更高。这可能是疾病相关菌株对频繁抗菌治疗的一种适应。
