C57BL/6J受体小鼠X射线照射及表达mTomato的骨髓细胞移植方案。

Protocol for X-ray irradiation of C57BL/6J recipient mice followed by the transplantation of mTomato-expressing bone marrow cells.

作者信息

Vo Thao, Kumar Rahul, Lamichhane Richa, Choudhary Ishita, Kc Rekha, Mao Yun, Paudel Kshitiz, Suryawanshi Amol, Sharma Amit, Metzler Marnie, Nolan Michael, Patial Sonika, Saini Yogesh

机构信息

Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.

Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA; Office of Research and Innovation, North Carolina State University, Raleigh, NC 27606, USA.

出版信息

STAR Protoc. 2025 Mar 21;6(1):103504. doi: 10.1016/j.xpro.2024.103504. Epub 2024 Dec 18.

Bone marrow chimera is a useful tool to determine the pathophysiological contributions of hematopoietic versus stromal compartments. Here, we present a protocol for lethal irradiation of wild-type C57BL/6J recipient mice followed by the transplantation of bone marrow from mTomato-expressing donors. We then detail procedures for animal distress scoring and assessment of reconstitution efficiency. This protocol will minimize distress and ensure efficient hematopoietic reconstitution of donor cells in recipients.