Li Yuxiao, Zhou Jie, Guo Tong, Zhang Huiya, Cao Chang, Cai Yingjie, Zhang Jiqiao, Li Tao, Zhang Jianqin
College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.
College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.
Poult Sci. 2025 Jan;104(1):104506. doi: 10.1016/j.psj.2024.104506. Epub 2024 Nov 10.
The use of bio-enzyme as feed additives holds significant potential. This study aimed to evaluate the impact of a kind of compound bio-enzyme supplementation (the main functional components are probiotics and astragalus polysaccharides) on the production performance, serum immunity, and intestinal health of Pekin ducks. A total of 126 male Pekin ducks were randomly assigned to three groups: a control group (CG, no additive), a low-dose group (LG, 0.1 % bio-enzyme), and a high-dose group (HG, 0.2 % bio-enzyme), with 6 replicates per group. Ducks were raised until 35 days of age, with weekly measurements of growth performance. At day 35, serum immunoglobulins were measured, carcass traits were recorded, and cecal contents were analyzed using 16S rRNA sequencing and metabolomics. Results indicated a significant increase in ADG (P = 0.049) and a decrease in feed-to-gain ratio (F:G) (P = 0.020) in LG and HG compared to CG during rearing. The HG showed a notable improvement in half eviscerated yield (HEY) (P = 0.023) and full eviscerated yield (FEY) (P = 0.008). No substantial changes were observed in immunological parameters (P > 0.05). The jejunal villus height to crypt depth ratio (VH/CD) significantly increased (P < 0.001) in LG, with notable improvements in duodenal (P = 0.001) and jejunal (P < 0.001) VH/CD in HG. The Shannon index (P = 0.042) and Pielou index (P = 0.038) of cecal microbiota were markedly lower in HG. Notable changes in the relative abundance of Firmicutes and Bacteroidota were observed in LG and HG. Differential bacteria and metabolites among the treatments were identified, and their correlations were analyzed. KEGG enrichment pathways of the metabolites were also identified. In conclusion, this bio-enzyme can improve production performance, intestinal wall structure, and microbiota in Pekin ducks. A 0.1 % concentration of this bio-enzyme is optimal for Pekin duck production.
使用生物酶作为饲料添加剂具有巨大潜力。本研究旨在评估一种复合生物酶添加剂（主要功能成分是益生菌和黄芪多糖）对北京鸭生产性能、血清免疫力和肠道健康的影响。总共126只雄性北京鸭被随机分为三组：对照组（CG，不添加）、低剂量组（LG，0.1%生物酶）和高剂量组（HG，0.2%生物酶），每组6个重复。鸭子饲养至35日龄，每周测量生长性能。在第35天，测量血清免疫球蛋白，记录屠体性状，并使用16S rRNA测序和代谢组学分析盲肠内容物。结果表明，在饲养期间，与对照组相比，LG和HG组的平均日增重（ADG）显著增加（P = 0.049），料重比（F:G）降低（P = 0.020）。HG组的半净膛率（HEY）（P = 0.023）和全净膛率（FEY）（P = 0.008）有显著改善。免疫参数未观察到实质性变化（P > 0.05）。LG组空肠绒毛高度与隐窝深度比值（VH/CD）显著增加（P < 0.001），HG组十二指肠（P = 0.001）和空肠（P < 0.001）的VH/CD有显著改善。HG组盲肠微生物群的香农指数（P = 0.042）和皮洛指数（P = 0.038）显著较低。LG和HG组中厚壁菌门和拟杆菌门的相对丰度有显著变化。确定了处理组之间的差异细菌和代谢物，并分析了它们的相关性。还确定了代谢物的KEGG富集途径。总之，这种生物酶可以改善北京鸭的生产性能、肠壁结构和微生物群。这种生物酶0.1%的浓度对北京鸭生产最为适宜。
