Liu Wen-Ze, Zhou Sheng-Wei, Zhang Shao-Ke, Wang Liu-Ming, Gu Xu-Peng, Chu Lei-Xia, Qiao Lu, Wan Jie, Zhang Xiao, Yang Lin-Lin, Dong Cheng-Ming, Feng Wei-Sheng
School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China.
School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.
Zhongguo Zhong Yao Za Zhi. 2024 Nov;49(21):5843-5855. doi: 10.19540/j.cnki.cjcmm.20240815.101.
Based on whole genome data, the identification and expression pattern analysis of the Atropa belladonna WRKY transcription factor family were conducted to provide a theoretical foundation for studying the biological functions and mechanisms of these transcription factors. In this study, bioinformatics methods were employed to identify members of the A. belladonna WRKY gene family and to predict their physicochemical properties, conserved motifs, promoter cis-acting elements, and chromosomal localization. Additionally, the expression patterns of the A. belladonna WRKY gene family under the regulation of environmental factors such as light quality and temperature were analyzed. The results revealed a total of 28 AbWRKY transcription factors, randomly distributed across 16 chromosomes, encoding 324-707 amino acids. Most AbWRKY proteins were acidic, unstable, and hydrophilic. Based on multiple sequence alignment and phylogenetic analysis, the WRKY gene family members were classified into two subfamilies. Conserved motif and domain analysis indicated that WRKY transcription factors in the same subfamily possessed conserved structural features. Promoter analysis predicted that the A. belladonna WRKY family contained light-responsive elements, hormone-responsive elements, and stress-responsive elements. Collinearity analysis showed that AbWRKY24 plays a crucial role in the expansion of the AbWRKY gene family. Then qRT-PCR results indicated that AbWRKY6, AbWRKY8, AbWRKY14, and AbWRKY24 responded to red light stress, while AbWRKY8, AbWRKY14, and AbWRKY24 responded to yellow light/low-temperature combined stress. AbWRKY6 and AbWRKY8 were significantly expressed in leaves and stems, AbWRKY27 and AbWRKY28 were significantly expressed in fibrous roots, and AbWRKY25 was significantly expressed in flowers. This study is the first to identify and analyze the WRKY gene family in A. belladonna and to examine its expression patterns under light and temperature regulation, laying a foundation for in-depth analysis and functional validation of the molecular mechanisms of A. belladonna WRKY transcription factors in responding to light quality and temperature environmental factors.
基于全基因组数据，开展了颠茄WRKY转录因子家族的鉴定及表达模式分析，为研究这些转录因子的生物学功能及机制提供理论基础。本研究采用生物信息学方法鉴定颠茄WRKY基因家族成员，并预测其理化性质、保守基序、启动子顺式作用元件及染色体定位。此外，还分析了颠茄WRKY基因家族在光质和温度等环境因子调控下的表达模式。结果显示，共鉴定出28个AbWRKY转录因子，随机分布于16条染色体上，编码324 - 707个氨基酸。多数AbWRKY蛋白呈酸性、不稳定且亲水性强。基于多序列比对和系统发育分析，WRKY基因家族成员被分为两个亚家族。保守基序和结构域分析表明，同一亚家族的WRKY转录因子具有保守的结构特征。启动子分析预测，颠茄WRKY家族含有光响应元件、激素响应元件和胁迫响应元件。共线性分析表明，AbWRKY24在AbWRKY基因家族的扩张中起关键作用。随后的qRT-PCR结果表明，AbWRKY6、AbWRKY8、AbWRKY14和AbWRKY24对红光胁迫有响应，而AbWRKY8、AbWRKY14和AbWRKY24对黄光/低温复合胁迫有响应。AbWRKY6和AbWRKY8在叶和茎中显著表达，AbWRKY27和AbWRKY28在须根中显著表达，AbWRKY25在花中显著表达。本研究首次鉴定并分析了颠茄中的WRKY基因家族，并研究了其在光和温度调控下的表达模式，为深入分析和功能验证颠茄WRKY转录因子响应光质和温度环境因子的分子机制奠定了基础。
