基于混合分组分析法测序鉴定花生（Arachis hypogaea L.）网斑病抗性的两个数量性状基因座
Identification of two QTLs for web blotch resistance in peanut (Arachis hypogaea L.) based on BSA-seq.
作者信息
Zhao Mingbo, Sun Ziqi, Qi Feiyan, Liu Hua, Pavan Stefano, Fu Liuyang, Wang Juan, Chen Guoquan, Zeng Fanpei, Liu Hongfei, Wu Xiaohui, Qu Pengyu, Dong Wenzhao, Zheng Zheng, Zhang Xinyou
机构信息
School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China.
Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Genetic Improvement of Oil Crops, Postgraduate T&R Base of Zhengzhou University, Zhengzhou, 450002, China.
出版信息
BMC Plant Biol. 2024 Dec 19;24(1):1193. doi: 10.1186/s12870-024-05930-8.
BACKGROUND
Peanut (Arachis hypogaea L.) is a globally important oilseed and cash crop. Web blotch is one of the most important peanut foliar diseases, causing severe yield losses worldwide.
RESULTS
In this study, an F population was used to identify quantitative trait loci (QTLs) for peanut web blotch resistance, based on bulked segregant analysis (BSA). Kompetitive Allele-Specific PCR (KASP) markers were developed and used to further narrow QTL intervals and detect candidate genes. Two major QTLs, qWBRA05 and qWBRA08 were identified, spanning physical intervals of 465.75 Kb and 434.83 Kb, and explaining percentages of phenotypic variation (PVE) of 8.79% and 15.09%, respectively. Moreover, two KASP markers were developed within the QTL interval effectively distinguished between web blotch resistance and web blotch susceptible materials.
CONCLUSIONS
The QTLs identified and two molecular markers closely linked to web blotch resistance were developed within the QTL interval, which are potentially valuable in peanut breeding.
背景
花生（Arachis hypogaea L.）是一种全球重要的油料作物和经济作物。网斑病是花生最重要的叶部病害之一，在全球范围内造成严重的产量损失。
结果
在本研究中，基于混合分组分析法（BSA），利用一个F群体鉴定花生抗网斑病的数量性状位点（QTL）。开发了竞争性等位基因特异性PCR（KASP）标记，并用于进一步缩小QTL区间和检测候选基因。鉴定出两个主要QTL，qWBRA05和qWBRA08，其物理区间分别为465.75 Kb和434.83 Kb，分别解释表型变异百分比（PVE）为8.79%和15.09%。此外，在QTL区间内开发的两个KASP标记能够有效区分抗网斑病材料和感网斑病材料。
结论
鉴定出的QTL以及在QTL区间内开发的与抗网斑病紧密连锁的两个分子标记，在花生育种中具有潜在价值。
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