Agricultural Research Service of the United States Department of Agriculture, Temperate Tree Fruit and Vegetable Research Unit, Prosser, WA 99350.
Agricultural Research Service of the United States Department of Agriculture, Grain Legume Genetics and Physiology Research Unit, Prosser, WA 99350.
Plant Dis. 2021 Sep;105(9):2503-2508. doi: 10.1094/PDIS-09-20-1917-RE. Epub 2021 Oct 17.
(PSbMV) is both seedborne and aphid-transmitted and can cause economic losses for pea ( L.) production by reducing yield through decreased seed weight and number. The P1 pathotype is especially virulent, affecting this important vegetable crop across the United States and internationally in regions of West Asia, North Africa, Europe, and Australia. Previously, two kompetitive allele-specific PCR (KASP) genotyping markers ( and ) were developed and validated on accessions identifying two PSbMV pathotype P1 resistance alleles in the eukaryotic translation initiation factor gene, . The current study utilized these novel markers to rapidly evaluate 318 genetic resource accessions maintained as part of the United States Department of Agriculture National Plant Germplasm System's Pea Single Plant Collection (PSPC). The evaluations also included 58 commercial and other plant introduction (PI) lines that were assessed for the two resistance alleles. All genotyping results were validated in greenhouse assays by confirmation of observable disease symptoms after inoculations and by enzyme-linked immunosorbent assays. The and alleles were found in 18 accessions from the PSPC, five commercial lines, and 14 other PI accessions. A single PSPC accession showed resistance to PSbMV pathotype P1 that is believed to be a novel source of resistance based on sequencing analysis of . Sources of resistance were identified in the PSPC and in commercial cultivars that can be introgressed into breeding lines using traditional techniques to reduce the time and cost required to generate germplasm with superior disease-resistant traits.
（PSbMV）既是种子传播的，也是蚜虫传播的，通过降低种子重量和数量来减少产量，从而对豌豆（L.）生产造成经济损失。P1 致病型特别具有毒性，影响美国各地和西亚、北非、欧洲和澳大利亚等地区的国际重要蔬菜作物。以前，开发并验证了两种竞争等位基因特异性 PCR（KASP）基因分型标记（和），用于鉴定真核翻译起始因子基因中的两个 PSbMV 致病型 P1 抗性等位基因（）中的 333 个豌豆资源访问。本研究利用这些新标记快速评估了作为美国农业部国家植物种质系统豌豆单株收集（PSPC）一部分保存的 318 个遗传资源访问。评估还包括 58 个商业和其他植物引种（PI）品系，用于评估这两个 抗性等位基因。所有基因分型结果均通过接种后观察到可观察到的疾病症状的确认和酶联免疫吸附测定在温室试验中进行验证。在 PSPC 中发现了 18 个访问者，在 5 个商业线和 14 个其他 PI 访问者中发现了 18 个访问者，在 PSPC 中发现了 18 个访问者，在 5 个商业线和 14 个其他 PI 访问者中发现了 18 个访问者。一个 PSPC 访问者对 PSbMV 致病型 P1 表现出抗性，这被认为是基于对 的测序分析的一种新的抗性来源。在 PSPC 和商业品种中发现了抗性来源，可以使用传统技术将抗性引入育种种质中，从而减少生成具有优良抗病性状的种质所需的时间和成本。
