Xu Yuncong, Yang Yuhui, Shi Yonghui, Li Bowen, Xie Yanli, Le Guowei
Henan Key Laboratory of cereal and Oil Food Safety Inspection and Control, College of Food Science and Engineering, Henan University of Technology, Zhengzhou, Henan, China.
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, China.
NPJ Sci Food. 2024 Dec 19;8(1):104. doi: 10.1038/s41538-024-00348-w.
To explore the effects of methionine (Met) supplementation on cognitive dysfunction and the associated mechanisms in aging mice. The mice were administrated 0.15 g/kg/day D-galactose subcutaneously and fed a normal (0.86% Met) or a Met-supplemented diet (1.72% Met) for 11 weeks. Behavioral experiments were conducted, and we measured the plasma metabolite levels, hippocampal and plasma redox and inflammatory states, and hippocampal transsulfuration pathway-related parameters. Met supplementation prevented aging-induced anxiety and cognitive deficiencies, and normalized the plasma levels of multiple systemic metabolites (e.g., betaine, taurine, and choline). Furthermore, dietary Met supplementation abolished oxidative stress and inflammation, selectively modulated the expression of multiple cognition-related genes and proteins, and increased flux via the transsulfuration pathway in the hippocampi of aging mice, with significant increase in HS and glutathione production. Our findings suggest that dietary Met supplementation prevented cognitive deficiencies in aging mice, probably because of increased flux via the transsulfuration pathway.
为探讨补充蛋氨酸（Met）对衰老小鼠认知功能障碍及相关机制的影响。给小鼠皮下注射0.15 g/kg/天的D-半乳糖，并给予正常饮食（0.86% Met）或补充Met的饮食（1.72% Met）11周。进行行为实验，我们测量了血浆代谢物水平、海马体和血浆的氧化还原及炎症状态，以及海马体转硫途径相关参数。补充Met可预防衰老引起的焦虑和认知缺陷，并使多种全身代谢物（如甜菜碱、牛磺酸和胆碱）的血浆水平正常化。此外，饮食中补充Met可消除氧化应激和炎症，选择性调节多种认知相关基因和蛋白质的表达，并增加衰老小鼠海马体中转硫途径的通量，同时显著增加同型半胱氨酸和谷胱甘肽的产生。我们的研究结果表明，饮食中补充Met可预防衰老小鼠的认知缺陷，可能是因为转硫途径通量增加。
