Uddin Md Nasir, Mia Md Arzo, Akter Yasmin, Chowdhury Mohammad Al-Baruni, Rahman Md Hadisur, Siddiqua Hafsa, Shathi Umme Salma, Al-Mamun Abdullah, Siddika Farida, Marzan Lolo Wal
Laboratory of Microbial Genomics and Metabolic Engineering, Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chattogram, Bangladesh.
Molecular Biotechnology Division, National Institute of Biotechnology, Savar, Bangladesh.
Bioinform Biol Insights. 2024 Dec 18;18:11779322241306388. doi: 10.1177/11779322241306388. eCollection 2024.
COVID-19 caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) had an adverse effect globally because it caused a global pandemic with several million deaths. This virus possesses spike protein that is cleaved or activated by Furin-like protease enzymes occurring by mammalian lung or respiratory cells to enter the mammalian body. The addition of the Furin cleavage site in SARS-CoV-2 makes it a more infectious and emerging virus than its ancestor's viruses. Phylogenetic relationships of coronavirus spike proteins have analyzed and mapped Furin recognition motif on the tree using bioinformatics tools such as GTEx, KEGG, GO, NCBI, PolyPhen-2, SNAP2, PANTHER, Hidden Markov Models (Fathmm), Phd-single-nucleotide polymorphism (SNP), I-TASSER, Modpred, Phobius, SIFT, iPTREE-STAB, and PROVEAN. During this study, it has been found that in certain regions, SNPs have some relation with the susceptibility to SARS-CoV-2. Whereas in other regions, the effects are very negligible. Finally, our study demonstrates that SNPs have a strong relationship with susceptibility to SARS-CoV-2. As it helps to cleave the spike protein of the virus, thus it can be targeted to inhibit at a particular site to prevent the SARS-CoV-2 from the entrance into the body.
由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的COVID-19在全球产生了不利影响，因为它引发了一场导致数百万人死亡的全球大流行。这种病毒拥有刺突蛋白，该蛋白可被哺乳动物肺或呼吸细胞中存在的弗林蛋白酶样蛋白酶切割或激活，从而进入哺乳动物体内。SARS-CoV-2中弗林蛋白酶切割位点的添加使其比其祖先病毒更具传染性且更易出现。利用GTEx、KEGG、GO、NCBI、PolyPhen-2、SNAP2、PANTHER、隐马尔可夫模型（Fathmm）、博士单核苷酸多态性（SNP）、I-TASSER、Modpred、Phobius、SIFT、iPTREE-STAB和PROVEAN等生物信息学工具，分析了冠状病毒刺突蛋白的系统发育关系并在树上绘制了弗林蛋白酶识别基序。在这项研究中，发现某些区域的单核苷酸多态性与对SARS-CoV-2的易感性有一定关系。而在其他区域，影响非常微不足道。最后，我们的研究表明单核苷酸多态性与对SARS-CoV-2的易感性有很强的关系。由于它有助于切割病毒的刺突蛋白，因此可以在特定位点进行靶向抑制，以防止SARS-CoV-2进入体内。
