Department of Animal Sciences, University of Illinois, Urbana, IL, 61801, USA.
Division of Nutritional Sciences, University of Illinois, Urbana, IL, 61801, USA.
Eur J Nutr. 2022 Feb;61(1):461-475. doi: 10.1007/s00394-021-02658-1. Epub 2021 Sep 3.
Determine digestible indispensable amino acid score (DIAAS) for animal- and plant-based burgers and test the hypothesis that DIAAS calculated for a burger and a burger bun is additive in a combined meal.
Ten ileal cannulated gilts were fed experimental diets for six 9-d periods with ileal digesta being collected on d 8 and 9 of each period. Six diets contained a burger (i.e., 80% lean beef, 93% lean beef, 80% lean pork, Impossible Burger, or Beyond Burger) or a burger bun as the sole source of crude protein and amino acids. Three additional diets were based on a combination of the bun and 80% beef, pork, or Impossible Burger. A nitrogen-free diet was also used. The DIAAS for all ingredients and mixed meals was calculated for children from 6 months to 3 years and for individuals older than 3 years, and DIAAS for combined meals was predicted from individual ingredient DIAAS.
The 93% lean beef and the pork burgers had greater (P < 0.05) DIAAS than the plant-based burgers for both age groups. The 80% lean beef burger had greater (P < 0.05) DIAAS than the plant burgers for children from 6 months to 3 years, and greater (P < 0.05) DIAAS than the Beyond Burger for individuals older than 3 years. There were no differences between the measured and predicted DIAAS.
The protein quality of animal-based burgers is greater than that of plant-based burgers. However, for individuals older than 3 years, the Impossible Burger has comparable protein quality to the 80% lean beef burger. The DIAAS obtained from individual foods is additive in mixed meals.
确定动物源和植物源汉堡的可消化必需氨基酸评分(DIAAS),并检验汉堡和汉堡面包组合膳食中 DIAAS 可加性的假设。
10 头回肠可插管的母猪接受 6 个 9 天期的实验日粮,每个时期的第 8 和第 9 天收集回肠食糜。6 种日粮仅包含汉堡(即 80%精瘦牛肉、93%精瘦牛肉、80%精瘦猪肉、Impossible Burger 或 Beyond Burger)或汉堡面包作为粗蛋白和氨基酸的唯一来源。另外 3 种日粮基于面包和 80%牛肉、猪肉或 Impossible Burger 的组合。还使用了无氮饮食。为 6 个月至 3 岁的儿童以及 3 岁以上的个体计算了所有成分和混合膳食的 DIAAS,并根据单个成分的 DIAAS 预测了组合膳食的 DIAAS。
对于两个年龄组,93%精瘦牛肉和猪肉汉堡的 DIAAS 均高于植物源汉堡(P<0.05)。对于 6 个月至 3 岁的儿童,80%精瘦牛肉汉堡的 DIAAS 高于植物汉堡(P<0.05),对于 3 岁以上的个体,DIAAS 高于 Beyond Burger(P<0.05)。实测和预测的 DIAAS 之间没有差异。
动物源汉堡的蛋白质质量优于植物源汉堡。然而,对于 3 岁以上的个体,Impossible Burger 的蛋白质质量与 80%精瘦牛肉汉堡相当。单独食物获得的 DIAAS 在混合膳食中是可加的。