Moriya O, Ichikawa Y
Dev Comp Immunol. 1982 Fall;6(4):717-26.
Almost pure eosinophils have been isolated from chicken bursa, spleen, thymus or blood using Ficoll metrizoate and Ficoll gradient (eosinophil p.c. 71.4-87.6). Centrifugation by Ficoll discontinuous gradient yielded clear cut interface layer and had no deleterious influence on eosinophil viability. Purified eosinophil populations were examined for eosinophil migration response in vitro by exposure of the eosinophil population to lymphocytes sensitized in ovo with dinitrophenylated bovine serum albumin (DNP-BSA), DNP or BSA. Embryonic lymphocytes sensitized with antigens used led a eosinophil migration in vitro. From these findings, it is suggested that immunological maturation of the lymphocytes in the production of chemotactic factor may occur during the developing stage of chick embryos by antigenic stimulation.
使用聚蔗糖泛影酸盐和聚蔗糖梯度，已从鸡法氏囊、脾脏、胸腺或血液中分离出几乎纯的嗜酸性粒细胞（嗜酸性粒细胞占比71.4 - 87.6%）。通过聚蔗糖不连续梯度离心产生了清晰的界面层，且对嗜酸性粒细胞的活力没有有害影响。通过将嗜酸性粒细胞群体暴露于用二硝基苯基化牛血清白蛋白（DNP - BSA）、DNP或BSA在卵内致敏的淋巴细胞，来体外检测纯化的嗜酸性粒细胞群体的嗜酸性粒细胞迁移反应。用所用抗原致敏的胚胎淋巴细胞在体外导致嗜酸性粒细胞迁移。从这些发现表明，淋巴细胞在趋化因子产生中的免疫成熟可能在鸡胚胎发育阶段通过抗原刺激而发生。
