Cheng Yan, Zhang Tianxi, Yang Chao, Gebeyew Kefyalew, Ye Chengyu, Zhou Xinxin, Zhang Tianqi, Feng Ganyi, Li Rui, He Zhixiong, Parnas Oren, Tan Zhiliang
CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, 410125, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Commun Biol. 2024 Dec 20;7(1):1686. doi: 10.1038/s42003-024-07406-9.
Unlike monogastric animals, ruminants exhibit significantly lower starch digestibility in the small intestine. A better understanding of the physiological mechanisms that regulate digestion patterns in ruminants could lead to an increased use of starch concentrates. Here we show more robust pancreatic exocrine function in adult goats (AG) than in neonatal goats (NG) by combining scRNA-seq and proteomic analysis. Our findings suggest that inadequate amylase activity could be a limiting factor in starch digestion in ruminants. In addition, we show that insufficient starch hydrolysis in adult goats might be associated with low expression of a CCKBR receptor in the acinar cells. On top of that, the low expression of CCKBR in adult goats, in conjunction with a low distribution of the CCK-I cells in the duodenum, may jointly lead to a slow response of the intestinal-pancreatic reflex and induce an asynchronous process of food entering the small intestine and releasing of digestive enzymes, which ultimately limits the starch digestibility. Overall, the present findings generate a resource that can provide better insight into the mammalian pancreas.
与单胃动物不同，反刍动物在小肠中的淀粉消化率显著较低。更好地了解调节反刍动物消化模式的生理机制可能会增加淀粉浓缩物的使用。在这里，我们通过结合单细胞RNA测序和蛋白质组分析表明，成年山羊（AG）的胰腺外分泌功能比新生山羊（NG）更强健。我们的研究结果表明，淀粉酶活性不足可能是反刍动物淀粉消化的限制因素。此外，我们表明成年山羊淀粉水解不足可能与腺泡细胞中CCKBR受体的低表达有关。除此之外，成年山羊CCKBR的低表达，连同十二指肠中CCK-I细胞的低分布，可能共同导致肠-胰反射反应迟缓，并引发食物进入小肠和消化酶释放的不同步过程，最终限制淀粉消化率。总体而言，目前的研究结果提供了一种资源，可更好地洞察哺乳动物的胰腺。
