Nandi R, Maiti M
Biochem Pharmacol. 1985 Feb 1;34(3):321-4. doi: 10.1016/0006-2952(85)90038-3.
The binding of the alkaloid sanguinarine to natural DNAs of differing GC content has been studied by spectrophotometry and viscometry techniques. Binding parameters determined from spectrophotometric measurements by Scatchard analysis, according to an excluded-site model, indicate a very high specificity of sanguinarine binding to GC rich DNA. In the strong binding region, the increase of contour length of DNA depends strongly on its base composition, being larger with GC rich DNA than with AT rich DNA. It is concluded that the alkaloid binds preferentially to the GC pairs in DNA template.
通过分光光度法和粘度测定技术研究了生物碱血根碱与不同GC含量的天然DNA的结合。根据排阻位点模型，通过Scatchard分析从分光光度测量中确定的结合参数表明，血根碱与富含GC的DNA结合具有非常高的特异性。在强结合区域，DNA轮廓长度的增加强烈依赖于其碱基组成，富含GC的DNA比富含AT的DNA增加得更大。得出的结论是，生物碱优先与DNA模板中的GC碱基对结合。
