Li Xue, Liu Pei, Wang Zhaohui, Wei Xuefei, Gao Shuai, Fan YuChen, Liu Huihui, Wang Kai
Department of Hepatology, Qilu Hospital of Shandong University, Wenhuaxi Road 107#, Jinan, 250012, China.
Institute of Hepatology, Shandong University, Jinan, 250012, China.
Virol J. 2024 Dec 23;21(1):332. doi: 10.1186/s12985-024-02605-6.
Oxidative stress plays a crucial role in the pathogenesis of HBV. This study aimed to investigate the value of fibroblast growth factor 21 (FGF21) promoter methylation in the occurrence and development of chronic hepatitis B (CHB) oxidative stress.
A total of 241 participants including 221 patients with CHB and 20 healthy controls (HCs) were recruited. Methylation level of FGF21 promoter in peripheral blood mononuclear cells was quantitatively determined. Enzyme-linked immunosorbent assay was used to assess oxidative stress in CHB patients.
Our study shows that the FGF21 methylation level was significantly lower in HBeAg-positive CHB patients compared to HBeAg-negative CHB patients and HCs (P < 0.0001). The oxidative stress of HBeAg-positive CHB patients was more severe. Further correlation analysis showed that there was a significant correlation between the methylation level of FGF21 promoter and the occurrence of oxidative stress in CHB patients. In addition, assessment based on FGF21 promoter methylation level proved effective for predicting oxidative stress occurrence and disease progression among CHB patients.
FGF21 promoter methylation level is an important marker for predicting oxidative stress and disease progression in patients with CHB.
氧化应激在乙型肝炎病毒（HBV）发病机制中起关键作用。本研究旨在探讨成纤维细胞生长因子21（FGF21）启动子甲基化在慢性乙型肝炎（CHB）氧化应激发生发展中的价值。
共招募241名参与者，包括221例CHB患者和20名健康对照（HCs）。定量测定外周血单个核细胞中FGF21启动子的甲基化水平。采用酶联免疫吸附测定法评估CHB患者的氧化应激。
我们的研究表明，与HBeAg阴性CHB患者和HCs相比，HBeAg阳性CHB患者的FGF21甲基化水平显著降低（P < 0.0001）。HBeAg阳性CHB患者的氧化应激更严重。进一步的相关性分析表明，CHB患者中FGF21启动子甲基化水平与氧化应激的发生之间存在显著相关性。此外，基于FGF21启动子甲基化水平的评估被证明对预测CHB患者氧化应激的发生和疾病进展有效。
FGF21启动子甲基化水平是预测CHB患者氧化应激和疾病进展的重要标志物。
