Murali Jeeva, Rajendran Venkatesh, Balasubramanian Venkatrajah, Sampath Shobana, AlSalhi Mohamad S, Devanesan Sandhanasamy
Department of Microbiology, CMS College of Science and Commerce, Coimbatore, Tamil Nadu, India.
Department of Biochemistry, Kongunadu Arts and Science College, Coimbatore, Tamil Nadu, India.
Luminescence. 2024 Dec;39(12):e70070. doi: 10.1002/bio.70070.
The current study's initial goal was to investigate the anticancer activity of the extracts from the flowering plant Nyctanthes arbor-tristis. The ethanol extracts' phytochemical analysis was qualitatively determined. It was determined that the various phytochemical substances had antibacterial, antioxidant, and anticancer effects. GC-MS analysis revealed compounds like maleic anhydride, butanedioic acid, and oxalic acid emphasize the antioxidant and anticancer properties. MTT assay was investigated against three different cell lines (HepG2, A549, and MCF7). High level of resistance to HepG2 liver cancer cell lines was noted with ethanol flower extracts with IC value of 67.98 μg/mL. EtBr staining assay revealed HepG2 apoptotic cells displaying nuclear and membrane alterations when exposed to 70 μg/mL of ethanol extract. The cells treated with 70 μg/mL of extract showed a reduction of mitochondrial membrane potential, as evidenced by the MMP assay, which showed red fluorescence decreasing and green fluorescence increasing. DNA fragmentation analysis showed HepG2 cancer cells showed no signs of DNA fragmentation after being treated with 50 μM treatments of flower extracts for 72 h. Quantitative RT-PCR analysis showed increased Bcl2 gene expression with fold variation level of about 0.086094579. The obtained results emphasized the significance of Nyctanthes arbor-tristis extracts with a suggestion of chemotherapeutic medication to treat various cancers in the future.
当前研究的初始目标是调查开花植物夜花树提取物的抗癌活性。对乙醇提取物进行了植物化学定性分析。结果表明，各种植物化学物质具有抗菌、抗氧化和抗癌作用。气相色谱-质谱联用（GC-MS）分析显示，马来酸酐、丁二酸和草酸等化合物突出了其抗氧化和抗癌特性。针对三种不同的细胞系（HepG2、A549和MCF7）进行了MTT试验。乙醇花提取物对HepG2肝癌细胞系具有较高的耐药性，IC值为67.98μg/mL。溴化乙锭（EtBr）染色试验显示，当暴露于70μg/mL乙醇提取物时，HepG2凋亡细胞出现核和膜改变。用70μg/mL提取物处理的细胞显示线粒体膜电位降低；线粒体膜电位（MMP）试验证明了这一点，该试验显示红色荧光减少，绿色荧光增加。DNA片段化分析显示，用50μM花提取物处理72小时后，HepG2癌细胞没有DNA片段化迹象。定量逆转录-聚合酶链反应（RT-PCR）分析显示，Bcl2基因表达增加，倍数变化水平约为0.086094579。所得结果强调了夜花树提取物的重要性，并暗示其在未来可能成为治疗各种癌症的化疗药物。
