Department of Biotechnology, Kumaun University, Bhimtal Campus, Nainital, Uttarakhand, 263136, India.
Department of Chemistry, Kumaun University, DSB Campus, Nainital, Uttarakhand, 263001, India.
Appl Biochem Biotechnol. 2024 Jan;196(1):436-456. doi: 10.1007/s12010-023-04552-4. Epub 2023 May 4.
Nyctanthes arbor-tristis, alias "Vishnu Parijat," is a medicinal plant used to treat various inflammation-associated ailments and to combat innumerable infections in the traditional system of medicine. In the present study, we collected the samples of N. arbor-tristis from the lower Himalayan region of Uttarakhand, India, and carried out their molecular identification through DNA barcoding. To examine the antioxidant and antibacterial activities, we prepared the ethanolic and aqueous extracts (from flowers and leaves) and performed their phytochemical analysis by using different qualitative and quantitative approaches. The phytoextracts showed marked antioxidant potential, as revealed by a comprehensive set of assays. The ethanolic leaf extract showed marked antioxidant potential towards DPPH, ABTS, and NO scavenging (IC = 30.75 ± 0.006, 30.83 ± 0.002, and 51.23 ± 0.009 μg/mL, respectively). We used TLC-bioautography assay to characterize different antioxidant constituents (based on their Rf values) in the chromatograms ran under different mobile phases. For one of the prominent antioxidant spots in TLC bioautography, GC-MS analysis identified cis-9-hexadecenal and n-hexadecanoic acid as the major constituents. Furthermore, in antibacterial study, the ethanolic leaf extract showed marked activity against Aeromonas salmonicida (113.40 mg/mL of extract was equivalent to 100 μg/mL of kanamycin). In contrast, the ethanolic flower extract showed considerable antibacterial activity against Pseudomonas aeruginosa (125.85 mg/mL of extract ≡100 μg/mL of kanamycin). This study presents the phylogenetic account and unravels the antioxidant-related properties and antibacterial potential of N. arbor-tristis.
印度北阿坎德邦喜马偕尔山脚下的 Nyctanthes arbor-tristis（别名 Vishnu Parijat），是一种传统医学中用于治疗各种炎症相关疾病和对抗无数感染的药用植物。在本研究中，我们从印度北阿坎德邦的喜马偕尔山脚下地区收集了 N. arbor-tristis 的样本，并通过 DNA 条形码进行了分子鉴定。为了研究抗氧化和抗菌活性，我们制备了来自花和叶的乙醇和水提物，并通过使用不同的定性和定量方法进行了植物化学分析。植物提取物显示出显著的抗氧化潜力，这是通过一系列综合测试来证明的。乙醇叶提取物对 DPPH、ABTS 和 NO 清除表现出显著的抗氧化潜力（IC = 30.75 ± 0.006、30.83 ± 0.002 和 51.23 ± 0.009 μg/mL）。我们使用 TLC-生物自显影法根据不同移动相下运行的色谱图中的 Rf 值来表征不同的抗氧化成分。对于 TLC 生物自显影中一个突出的抗氧化点，GC-MS 分析鉴定出顺式-9-十六烯醛和正十六烷酸为主要成分。此外，在抗菌研究中，乙醇叶提取物对嗜水气单胞菌表现出显著的活性（113.40 mg/mL 提取物相当于 100 μg/mL 卡那霉素）。相比之下，乙醇花提取物对铜绿假单胞菌显示出相当大的抗菌活性（125.85 mg/mL 提取物≡100 μg/mL 卡那霉素）。本研究提供了 N. arbor-tristis 的系统发育记录，并揭示了其抗氧化相关特性和抗菌潜力。
