Bowen Ryan M, Lee Jake, Wang Brendon, Lohse Keith R, Miao Hanyang, Padawer-Curry Jonah A, Albertson Asher J, Landsness Eric C, Bauer Adam Q, Lee Jin-Moo
Washington University in St. Louis, Department of Neurology, St. Louis, Missouri, United States.
Washington University in St. Louis, Department of Biomedical Engineering, St. Louis, Missouri, United States.
Neurophotonics. 2025 Jan;12(Suppl 1):S14604. doi: 10.1117/1.NPh.12.S1.S14604. Epub 2024 Dec 20.
Stroke is the leading cause of chronic disability in the United States. How stroke size affects post-stroke repair and recovery is poorly understood.
We aim to investigate the effects of stroke size on early repair patterns and determine how early changes in neuronal circuits and networks predict functional outcomes after stroke.
We used wide-field optical imaging, photothrombosis, and the cylinder-rearing assay to examine changes in neuronal circuit and network activity in the context of functional recovery after stroke.
Larger strokes ablating caused diffuse and widespread forepaw stimulus-evoked cortical activation, including contralesional regions evolving within 4 weeks post-stroke; smaller strokes resulted in more focused ipsilesional activation. Larger strokes decreased neuronal fidelity and bilateral coherence during stimulation of either the affected or unaffected forepaw within this 4-week period. Mice in the larger lesion group demonstrated hyperconnectivity within the contralesional hemisphere at the resting state. Greater degrees of remapping diffusivity, neuronal fidelity degradation, and hyperconnectivity predicted worse 8-week recovery after statistically controlling for the effect of infarct size.
These results suggest that diffuse patterns of remapping, and desynchronization and hyperconnectivity of cortical networks, evolving early after stroke may reflect maladaptive plasticity, predicting poor long-term functional recovery.
中风是美国慢性残疾的主要原因。中风面积如何影响中风后的修复和恢复尚不清楚。
我们旨在研究中风面积对早期修复模式的影响，并确定神经元回路和网络的早期变化如何预测中风后的功能结果。
我们使用宽视野光学成像、光血栓形成和圆筒饲养试验来检查中风后功能恢复背景下神经元回路和网络活动的变化。
较大面积的中风消融导致弥漫性和广泛的前爪刺激诱发的皮质激活，包括中风后4周内对侧区域的演变；较小面积的中风导致更集中的同侧激活。在这4周内，较大面积的中风在刺激受影响或未受影响的前爪时会降低神经元保真度和双侧连贯性。较大损伤组的小鼠在静息状态下对侧半球表现出过度连接。在对梗死面积的影响进行统计控制后，更大程度的重映射扩散、神经元保真度下降和过度连接预示着8周恢复情况更差。
这些结果表明，中风后早期出现的弥漫性重映射模式以及皮质网络的去同步化和过度连接可能反映了适应性不良的可塑性，预示着长期功能恢复不佳。
