Aamer Helmy A, Elalem Saad F, Al-Askar Abdulaziz A, Sharaf Omaima A, Gaber Mahmoud A, Kowalczewski Przemysław, Behiry Said, Abdelkhalek Ahmed
Department of Chemistry and Technology of Pesticide, Agriculture Faculty (El-Shatby), Alexandria University, Alexandria, 21545, Egypt.
College of Physical Education and Sport Sciences, Al-Mustaqbal University, Babylon, Iraq.
Open Life Sci. 2024 Dec 16;19(1):20221011. doi: 10.1515/biol-2022-1011. eCollection 2024.
Methanolic extract from was investigated for its phytochemical content, antioxidant, and antimicrobial properties against phytopathogenic fungi and bacteria. Phytochemical analysis revealed the presence of saponin, tannins, and alkaloids with 1.25%, 18.8 mg catechin/g of extract, and 9.12%, respectively. Total flavonoid content was 20.8 mg quercetin equivalent/g while total phenolic content was 202 mg gallic acid equivalent/g. Antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay resulted in an IC value of 48.61 µg/mL, while the phosphomolybdenum method yielded a value of 215.43 mg ascorbic acid equivalent/g of extract. The highest phenolic acids detected in the extract were gallic acid (712.97 µg/g), syringic acid (742.7 µg/g), and caffeic acid (474.70 µg/g) according to high-performance liquid chromatography analysis. Palmitic acid (28.38%) dominated the fatty acids identified by gas chromatography-mass spectrometry, while stigmasterol (8.34%) was the most abundant steroid. At a concentration of 3 mg/mL, the extract showed strong antibacterial activity against (10.50 mm), (9.93 mm), and (8.37 mm). Additionally, the extract significantly suppressed fungal growth of (38.22%) and (33.56%) but showed lower activity toward (13.33%) at 5 mg/mL. In conclusion, extract exhibited promising antioxidant and antimicrobial properties, making it a potential candidate for further exploration in agricultural applications.
对[植物名称]的甲醇提取物进行了植物化学成分、抗氧化和抗植物病原真菌及细菌的抗菌性能研究。植物化学分析表明，提取物中存在皂苷、单宁和生物碱，含量分别为1.25%、18.8毫克儿茶素/克提取物和9.12%。总黄酮含量为20.8毫克槲皮素当量/克，总酚含量为202毫克没食子酸当量/克。采用2,2-二苯基-1-苦基肼法测定的抗氧化活性IC值为48.61微克/毫升，而磷钼酸法测定的值为215.43毫克抗坏血酸当量/克提取物。根据高效液相色谱分析，提取物中检测到的最高酚酸为没食子酸（712.97微克/克）、丁香酸（742.7微克/克）和咖啡酸（474.70微克/克）。气相色谱-质谱法鉴定的脂肪酸中，棕榈酸（28.38%）占主导地位，而豆甾醇（8.34%）是最丰富的类固醇。在浓度为3毫克/毫升时，提取物对[细菌名称1]（抑菌圈直径10.50毫米）、[细菌名称2]（9.93毫米）和[细菌名称3]（8.37毫米）表现出较强的抗菌活性。此外，在5毫克/毫升时，提取物显著抑制了[真菌名称1]（38.22%）和[真菌名称2]（33.56%）的真菌生长，但对[真菌名称3]的活性较低（13.33%）。总之，[植物名称]提取物表现出有前景的抗氧化和抗菌性能，使其成为农业应用中进一步探索的潜在候选物。
