Bashir Shimaa, Behiry Said, Al-Askar Abdulaziz A, Kowalczewski Przemysław Łukasz, Emaish Haitham H, Abdelkhalek Ahmed
Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, Alexandria, 21934, Egypt.
Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria, 21531, Egypt.
Open Life Sci. 2024 Sep 3;19(1):20220962. doi: 10.1515/biol-2022-0962. eCollection 2024.
The research into the use of plants as plentiful reservoirs of bioactive chemicals shows significant potential for agricultural uses. This study focused on analyzing the chemical composition and potency of an ethanolic extract obtained from the aerial parts (leaves and stems) of against potato pathogenic fungal and bacterial pathogens. The isolated fungal isolates were unequivocally identified as and based on morphological characteristics and internal transcribed spacer genetic sequencing data. The antifungal activity of the extract revealed good inhibition efficacy against (60.4%) and weak activity against (11.1%) at a concentration of 5,000 µg/mL. The extract exhibited strong antibacterial activity, as evidenced by the significant inhibition zone diameter (mm) observed in all three strains of bacteria that were tested: (13.33), (9.00), and (9.33), at a concentration of 10,000 µg/mL. High-performance liquid chromatography analysis revealed the presence of several polyphenolic compounds (μg/g), with gallic acid (2942.8), caffeic acid (2110.2), cinnamic acid (1943.1), and chlorogenic acid (858.4) being the predominant ones. Quercetin and hesperetin were the predominant flavonoid components, with concentrations of 1110.3 and 1059.3 μg/g, respectively. Gas chromatography-mass spectrometry analysis revealed the presence of many bioactive compounds, such as saturated and unsaturated fatty acids, diterpenes, and phytosterols. The most abundant compound detected was -hexadecanoic acid, which accounted for 28.1%. The results emphasize the potential of extract as a valuable source of bioactive substances that possess good antifungal and antibacterial effects, which highlights its potential for many agricultural uses.
对植物作为生物活性化学物质丰富储存库的研究显示出在农业用途方面的巨大潜力。本研究聚焦于分析从[植物名称]地上部分（叶和茎）获得的乙醇提取物对马铃薯致病真菌和细菌病原体的化学成分及效力。基于形态特征和内部转录间隔区基因测序数据，分离出的真菌菌株被明确鉴定为[具体真菌名称1]和[具体真菌名称2]。提取物的抗真菌活性显示，在浓度为5000μg/mL时，对[具体真菌名称1]有良好的抑制效果（60.4%），对[具体真菌名称2]活性较弱（11.1%）。[植物名称]提取物表现出较强的抗菌活性，在浓度为10000μg/mL时，对所测试的三种细菌菌株[具体细菌名称1]（抑菌圈直径13.33mm）、[具体细菌名称2]（抑菌圈直径9.00mm）和[具体细菌名称3]（抑菌圈直径9.33mm）均观察到明显的抑菌圈直径。高效液相色谱分析显示存在多种多酚类化合物（μg/g），其中没食子酸（2942.8）、咖啡酸（2110.2）、肉桂酸（1943.1）和绿原酸（858.4）为主要成分。槲皮素和橙皮素是主要的黄酮类成分，浓度分别为1110.3和1059.3μg/g。气相色谱 - 质谱分析显示存在许多生物活性化合物，如饱和与不饱和脂肪酸、二萜类和植物甾醇。检测到的最丰富化合物是十六烷酸（占28.1%）。结果强调了[植物名称]提取物作为具有良好抗真菌和抗菌作用的生物活性物质宝贵来源的潜力，突出了其在众多农业用途中的潜力。
