Christov K
Cell Tissue Kinet. 1985 Mar;18(2):119-31. doi: 10.1111/j.1365-2184.1985.tb00640.x.
The proliferation kinetics and DNA content of thyroid follicular cells in rats were studied by autoradiography and cytophotometry. Continuous treatment of animals with methylthiouracil (MTU) results in hyperplasia followed by tumour growth in the thyroid gland. The mitotic index (MI) increases from 0.006 +/- 0.002% in controls to 0.13 +/- 0.06% in hyperplasia and to 0.09 +/- 0.03% in malignant cells. The same is true for the labelling index (LI) which rises from 0.08 +/- 0.003% in controls to 1.4 +/- 1.1% in hyperplasia and to 1.0 +/- 0.6% in follicular adenomas. The S-phase duration (TS) is shortened from 8.0 +/- 1.2 hr in controls to 6.0 +/- 1.4 hr in animals treated for 9 months with MTU and prolonged to 15.4 +/- 2.1 hr in papillary carcinomas. In all MTU-treated animals a decrease in the value of the potential population doubling time (TPD) and thyroid weight doubling time (TD) was observed. The cell loss factor (phi) decreases in animals treated for 3 months with MTU and increases during the stage of tumour growth in the gland (animals treated 12-15 months with MTU). DNA measurements in the nuclei of hyperplastic and neoplastic thyroid tissues reveal cells with values exceeding that in control animals. However, no difference was found in the DNA content between thyroid adenomas and carcinomas, nor between thyroid hyperplasia and neoplasia.
采用放射自显影术和细胞光度测定法研究了大鼠甲状腺滤泡细胞的增殖动力学和DNA含量。用甲基硫氧嘧啶（MTU）持续处理动物会导致甲状腺增生，随后出现肿瘤生长。有丝分裂指数（MI）从对照组的0.006±0.002%增加到增生期的0.13±0.06%，再到恶性细胞的0.09±0.03%。标记指数（LI）也是如此，从对照组的0.08±0.003%增加到增生期的1.4±1.1%，再到滤泡性腺瘤的1.0±0.6%。S期持续时间（TS）从对照组的8.0±1.2小时缩短到用MTU处理9个月的动物的6.0±1.4小时，而在乳头状癌中延长到15.4±2.1小时。在所有用MTU处理的动物中，观察到潜在群体倍增时间（TPD）和甲状腺重量倍增时间（TD）的值降低。在用MTU处理3个月的动物中细胞丢失因子（phi）降低，而在甲状腺肿瘤生长阶段（用MTU处理12 - 15个月的动物）增加。对增生性和肿瘤性甲状腺组织细胞核中的DNA测量显示，其值超过对照动物。然而，甲状腺腺瘤和癌之间以及甲状腺增生和肿瘤之间的DNA含量没有差异。
