Matsushima Y, Kanzawa F, Hoshi A, Shimizu E, Nomori H, Sasaki Y, Saijo N
Cancer Chemother Pharmacol. 1985;14(2):104-7. doi: 10.1007/BF00434345.
To analyze the discrepancy between the in vitro response in the clonogenic assay and the clinical response, the time-schedule dependencies of various anticancer drugs were determined by comparing the inhibiting effect against colony formation by PC-7 cells treated with the drugs for 1 h with that of those treated for 24 h. According to their schedule dependency the drugs can be divided into a schedule-dependent drug group (5-fluorouracil, methotrexate, bleomycin, pepleomycin, etoposide, cisplatin, teniposide, vindesine, and vinblastine) and a non-schedule-dependent drug group (adriamycin, actinomycin D, ranomustine, mitomycin C, aclacinomycin, daunomycin, nimustine, melphalan, and KW 2083). In the clonogenic assay, the 1-h exposure schedule is appropriate for predicting clinical response for the non-schedule-dependent drugs. However, the effect of the schedule-dependent drugs was underestimated in the same conditions. Therefore, it is necessary to test these drugs in the assay by 24-h exposure for a more accurate assessment of their antitumor activity.
为分析克隆形成试验中的体外反应与临床反应之间的差异，通过比较用药物处理1小时的PC-7细胞与处理24小时的PC-7细胞对集落形成的抑制作用，确定了各种抗癌药物的时间依赖性。根据其时间依赖性，药物可分为时间依赖性药物组（5-氟尿嘧啶、甲氨蝶呤、博来霉素、培普利欧霉素、依托泊苷、顺铂、替尼泊苷、长春地辛和长春碱）和非时间依赖性药物组（阿霉素、放线菌素D、司莫司汀、丝裂霉素C、阿克拉霉素、柔红霉素、尼莫司汀、美法仑和KW 2083）。在克隆形成试验中，1小时暴露方案适用于预测非时间依赖性药物的临床反应。然而，在相同条件下，时间依赖性药物的效果被低估了。因此，有必要在试验中对这些药物进行24小时暴露测试，以更准确地评估其抗肿瘤活性。
