Jia Xueyan, Li Yang, Chen Lang, Xiao Yexin, Yang Ning, Luo Hongyuan, Guan Jing, Xu Delin
Department of Medical Instrumental Analysis, Zunyi Medical University, Zunyi, Guizhou 563099, China; Department of Cell Biology, Zunyi Medical University, Zunyi, Guizhou 563099, China.
Department of Cell Biology, Zunyi Medical University, Zunyi, Guizhou 563099, China.
Fitoterapia. 2025 Mar;181:106356. doi: 10.1016/j.fitote.2024.106356. Epub 2024 Dec 22.
Bletilla striata (Thunb.) Reichb.f is renowned for its traditional medicinal applications and a spectrum of pharmacological activities, which is intricately linked to militarine. Addressing sustainable production of B. striata and militarine necessitates innovative strategies. Endophytic fungi, residing within plant tissues and establishing symbiotic relationships, act as secondary genomes of plants, co-regulating plant growth and secondary metabolite synthesis. Despite their potential, the genetic and metabolic diversity, functional activity, and regulatory interactions of endophytic fungi with B. striata remain unexplored. This study aims to bridge this gap by investigating endophytic fungi that could enhance B. striata growth and militarine biosynthesis. The study revealed that endophytic fungi from pseudobulbs, roots, and stems were co-cultured with callus tissue of B. striata, and it was discovered that Serendipita indica from the Serendipita genus can enhance militarine accumulation. Subsequently, key genes, core enzymes, and regulatory factors related to militarine biosynthesis in the S. indica genome were analyzed. By employing advanced biotechnological and comparative genomic approaches, we elucidated the composition and distribution of regulatory factors across different endophytic fungal genomes associated with B. striata. This research not only advances our understanding of the symbiotic relationship between B. striata and its endophytic fungi but also provides a foundational blueprint for the sustainable exploitation and enhancement of militarine production.
白及（Bletilla striata (Thunb.) Reichb.f）以其传统药用价值和一系列药理活性而闻名，这与白及苷密切相关。解决白及和白及苷的可持续生产问题需要创新策略。内生真菌存在于植物组织内并建立共生关系，可作为植物的次生基因组，共同调节植物生长和次生代谢产物合成。尽管内生真菌具有潜力，但其与白及的遗传和代谢多样性、功能活性以及调控相互作用仍未得到探索。本研究旨在通过调查能够促进白及生长和白及苷生物合成的内生真菌来填补这一空白。研究发现，将来自假鳞茎、根和茎的内生真菌与白及愈伤组织共培养，发现丝核菌属的印度丝核菌（Serendipita indica）可增强白及苷积累。随后，分析了印度丝核菌基因组中与白及苷生物合成相关的关键基因、核心酶和调控因子。通过采用先进的生物技术和比较基因组学方法，我们阐明了与白及相关的不同内生真菌基因组中调控因子的组成和分布。本研究不仅增进了我们对白及与其内生真菌共生关系的理解，还为白及苷生产的可持续开发和提高提供了基础蓝图。
