Barthe Manon, Clerbaux Laure-Alix, Thénot Jean-Paul, Braud Véronique M, Osman-Ponchet Hanan
Laboratoires PKDERM, Grasse, France.
Institut de Pharmacologie Moléculaire et Cellulaire, Université Côte d'Azur, CNRS UMR7275, INSERM U1323, Valbonne, France.
Front Med (Lausanne). 2024 Dec 4;11:1481645. doi: 10.3389/fmed.2024.1481645. eCollection 2024.
The skin barrier plays a crucial role in protecting our body against external agents. Disruption of this barrier's function leads to increased susceptibility to infections and dermatological diseases. Damaged skin can be due to the use of detergents, sunburn or excessive scratching. In the context of the COVID-19 pandemic the recommended hygiene measures to prevent the spread of SARS-CoV-2, such as wearing masks, frequent handwashing, and the use of sanitizers, can also potentially alter the skin barrier.
The purpose of the study was to characterize the barrier function of models of damaged human skin.
Skin barrier damage was induced through different chemical and mechanical treatments, representative of the potential factors damaging human skin. The skin barrier function was evaluated in terms of permeability, dermal absorption capacity, thickness and gene expression of barrier markers. As inflammation is linked to skin barrier integrity, inflammatory markers were also analyzed.
The different treatments applied to skin models allow the simulation of diverse degrees of skin damage, making these models valuable for assessing the efficacy of topical products targeted at skin repair and for studying the effects of compromised skin barrier on viral penetration.
皮肤屏障在保护我们的身体免受外界因素侵害方面起着至关重要的作用。这种屏障功能的破坏会导致对感染和皮肤病的易感性增加。皮肤受损可能是由于使用洗涤剂、晒伤或过度抓挠。在新冠疫情背景下，为防止严重急性呼吸综合征冠状病毒2（SARS-CoV-2）传播而推荐的卫生措施，如戴口罩、勤洗手和使用消毒剂，也可能潜在地改变皮肤屏障。
本研究的目的是表征受损人类皮肤模型的屏障功能。
通过不同的化学和机械处理诱导皮肤屏障损伤，这些处理代表了损害人类皮肤的潜在因素。从渗透性、真皮吸收能力、厚度和屏障标志物的基因表达方面评估皮肤屏障功能。由于炎症与皮肤屏障完整性有关，还分析了炎症标志物。
应用于皮肤模型的不同处理能够模拟不同程度的皮肤损伤，使得这些模型对于评估针对皮肤修复的局部产品的疗效以及研究受损皮肤屏障对病毒渗透的影响具有重要价值。
