Zarei Parvin, Amirpour-Najafabadi Behnam, Sam-Sani Parnian, Sakhaie Mohammad Hassan, Sadegh Mehdi
Department of Bioinformatics, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
Adv Biomed Res. 2024 Oct 28;13:93. doi: 10.4103/abr.abr_178_23. eCollection 2024.
We aimed to investigate the effects of whey protein (WP) supplements in a rat model of rotenone-induced locomotor and biochemical features of Parkinson's disease (PD).
Male Wistar rats were used. Daily injections of rotenone (2 mg/kg; i.p.) for 16 days were used to induce PD. WP or soy protein (SP) at 1, 2, and 4 g/rats were administrated daily by gavage. Motor skills were measured in rats 24 h after the last injection using the bar test, grid test, rearing, and open field tests. In the following, striatum tissue was isolated for biochemical measurements. ELISA kits were used for biochemical assessments.
While rotenone caused a significant increase in the delay time in both the bar and grid tests and a significant decrease in the motor activities were observed in both rearing and spontaneous movement tests in the rotenone group, supplementation with 2 and 4 g of WP, but not SP, significantly decreased the delay time in the bar and grid tests and also significantly increased both rearing and spontaneous movements. Additionally, rotenone caused a significant decrease in striatal levels of dopamine and glutathione and significantly increased apoptotic caspases 8, 9, and Cytochrome C, while 2 and 4 g of WP, but not SP, significantly reversed these effects.
WP appears to have neuroprotective effects against rotenone-induced neurotoxicity and motor dysfunction, so it may be effective in the control of PD.
我们旨在研究乳清蛋白（WP）补充剂对鱼藤酮诱导的帕金森病（PD）大鼠模型运动和生化特征的影响。
使用雄性Wistar大鼠。每天腹腔注射鱼藤酮（2mg/kg），持续16天以诱导PD。每天通过灌胃给予大鼠1、2和4g的WP或大豆蛋白（SP）。在最后一次注射后24小时，使用杆式试验、网格试验、竖立体位试验和旷场试验测量大鼠的运动技能。随后，分离纹状体组织进行生化测量。使用酶联免疫吸附测定试剂盒进行生化评估。
鱼藤酮组在杆式试验和网格试验中的延迟时间显著增加，在竖立体位试验和自发运动试验中观察到运动活动显著减少，而补充2g和4g的WP而非SP可显著缩短杆式试验和网格试验中的延迟时间，并显著增加竖立体位运动和自发运动。此外，鱼藤酮导致纹状体中多巴胺和谷胱甘肽水平显著降低，凋亡半胱天冬酶8、9和细胞色素C显著增加，而2g和4g的WP而非SP可显著逆转这些作用。
WP似乎对鱼藤酮诱导的神经毒性和运动功能障碍具有神经保护作用，因此可能对控制PD有效。
