Siddique Yasir Hasan, Varshney Himanshi, Subhan Iqra, Gaur Kajal, Fatima Javeria, Jyoti Smita
Laboratory of Alternative Animal Models, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
Department of Zoology, School of Sciences, IFTM University, Moradabad, Uttar Pradesh, India.
Toxicol Rep. 2024 Nov 28;13:101839. doi: 10.1016/j.toxrep.2024.101839. eCollection 2024 Dec.
The effect of EMS at final concentration of 0.09, 0.18, 0.27 and 0.37 mM was studied on using morphological, regeneration, oxidative stress markers and DNA damage as parameters. The morphological scores showed a significant dose dependent difference in the exposed to 0.18, 0.27, and 0.37 mM of EMS for 24, 48, 72 and 96 h. The regeneration scores also showed a significant difference in the gastric region of exposed to 0.37 mM of EMS for 48 h. A significant difference in the scores of regeneration was observed for the mid body portion exposed to 0.18, 0.27 and 0.37 mM of EMS for 72 and 96 h of duration compared to control. A dose-dependent significant increase in the activities of glutathione-S-transferase (GST), catalase (CAT), and superoxide dismutase (SOD) was observed compared to control. The thiobarbituric acid reactive species (TBARS) levels were also significantly increased compared to control. The genotoxic damage was assessed in the cells of gastric region of the exposed to 0.09, 0.18, 0.27 and 0.37 mM of EMS for 48 h by performing comet assay. A significant dose-dependent increase in the DNA damage was observed compared to control.
研究了终浓度为0.09、0.18、0.27和0.37 mM的EMS对[实验对象]的影响，以形态学、再生、氧化应激标志物和DNA损伤为参数。形态学评分显示，在暴露于0.18、0.27和0.37 mM的EMS 24、48、72和96小时的[实验对象]中，存在显著的剂量依赖性差异。再生评分也显示，在暴露于0.37 mM的EMS 48小时的[实验对象]的胃部区域存在显著差异。与对照组相比，在暴露于0.18、0.27和0.37 mM的EMS 72和96小时的[实验对象]的身体中部，观察到再生评分存在显著差异。与对照组相比，观察到谷胱甘肽-S-转移酶（GST）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性呈剂量依赖性显著增加。硫代巴比妥酸反应性物质（TBARS）水平也比对照组显著增加。通过进行彗星试验，评估了暴露于0.09、0.18、0.27和0.37 mM的EMS 48小时的[实验对象]胃部区域细胞中的遗传毒性损伤。与对照组相比，观察到DNA损伤呈剂量依赖性显著增加。
