Division of Biotechnology, Manipal Life Sciences Centre, Manipal University, Planetarium, Complex, Manipal, Karnataka 576 104, India.
BMC Complement Altern Med. 2012 Aug 1;12:113. doi: 10.1186/1472-6882-12-113.
Ayurveda, the traditional Indian system of medicine has given great emphasis to the promotion of health. Rasayana is one of the eight branches of Ayurveda which refers to rejuvenant therapy. It has been reported that rasayanas have immuno-modulatory, antioxidant and antitumor functions, however, the genotoxic potential and modulation of DNA repair of many rasayanas have not been evaluated.
The present study assessed the role of Brahmarasayana (BR) on Ethyl methanesulfonate (EMS)-and Methyl methanesulfonate (MMS)-induced genotoxicity and DNA repair in in vivo mouse test system. The mice were orally fed with BR (5 g or 8 mg / day) for two months and 24 h later EMS or MMS was given intraperitoneally. The genotoxicity was analyzed by chromosomal aberrations, sperm count, and sperm abnormalities.
The results have revealed that BR did not induce significant chromosomal aberrations when compared to that of the control animals (p >0.05). On the other hand, the frequencies of chromosomal aberrations induced by EMS (240 mg / kg body weight) or MMS (125 mg / kg body weight) were significantly higher (p<0.05) to that of the control group. The treatment of BR for 60 days and single dose of EMS or MMS on day 61, resulted in significant (p <0.05) reduction in the frequency of chromosomal aberrations in comparison to EMS or MMS treatment alone, indicating a protective effect of BR. Constitutive base excision repair capacity was also increased in BR treated animals.
The effect of BR, as it relates to antioxidant activity was not evident in liver tissue however rasayana treatment was observed to increase constitutive DNA base excision repair and reduce clastogenicity. Whilst, the molecular mechanisms of such repair need further exploration, this is the first report to demonstrate these effects and provides further evidence for the role of brahmarasayana in the possible improvement of quality of life.
阿育吠陀，印度传统医学体系，非常重视健康促进。Rasayana 是阿育吠陀的八大分支之一，指的是 rejuvenant 疗法。据报道，rasayanas 具有免疫调节、抗氧化和抗肿瘤作用，但许多 rasayanas 的遗传毒性潜力和 DNA 修复调节尚未得到评估。
本研究评估了 Brahmarasayana（BR）在体内小鼠试验系统中对 Ethyl methanesulfonate（EMS）和 Methyl methanesulfonate（MMS）诱导的遗传毒性和 DNA 修复的作用。小鼠连续两个月口服 BR（5 g 或 8 mg/天），24 小时后腹腔内给予 EMS 或 MMS。通过染色体畸变、精子计数和精子异常分析遗传毒性。
结果表明，与对照组相比，BR 未诱导出明显的染色体畸变（p >0.05）。另一方面，EMS（240mg/kg 体重）或 MMS（125mg/kg 体重）诱导的染色体畸变频率明显高于对照组（p<0.05）。BR 处理 60 天，EMS 或 MMS 于第 61 天单次给药，与 EMS 或 MMS 单独处理相比，染色体畸变频率显著降低（p <0.05），表明 BR 具有保护作用。BR 处理还增加了基础切除修复能力。
BR 与抗氧化活性相关的作用在肝组织中不明显，但 rasayana 处理观察到增加了组成型 DNA 碱基切除修复并减少了断裂剂。虽然，这种修复的分子机制需要进一步探索，但这是首次报道证明这些效果，并为 brahmarasayana 在改善生活质量方面的作用提供了进一步证据。
