Zhang Xiumei, Qin Miao, Jia Jinqiao, Ahmed Akhlaq, Zhao Liqin, Lan Weiwei, Wei Yan, Liang Ziwei, Ma Xudong, Shi Yiwei, Huang Di
Research Center for Nano-Biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan 030024, China.
Research Center for Nano-Biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan 030024, China; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030032, China.
Int J Biol Macromol. 2025 Feb;290:139112. doi: 10.1016/j.ijbiomac.2024.139112. Epub 2024 Dec 22.
Adhesive hydrogels have been widely studied as wound dressings due to their excellent biocompatibility and biological activity. However, most designed hydrogels still exist limitations including potentially toxic monomer, complex preparation process and non-degradable property. Here, a natural and degradable gelatin/casein hydrogel was prepared by enzymatic cross-linking. The hydrogel could adhere to tissue on-demand with the mediation of chitosan (CS) solution. It was found that the adhesion strength of hydrogel could be controlled by adjusting gelatin/casein ratio, EDC&NHS concentration, CS concentration, glycerol content and crosslinking degree. To further expand the applicability of hydrogels, the degradation and drug release rate of hydrogels could be modulated by changing transglutaminase (TG) concentration. Moreover, tetracycline hydrochloride (TH) was loaded into hydrogel as a drug model, which endowed hydrogel with good antibacterial properties. It was shown that the 0.03 % TH hydrogel had excellent blood compatibility and cell compatibility, and can promote the healing of infected wounds in mice. This research provides a new natural adhesive hydrogel for biomedical engineering field.
由于具有优异的生物相容性和生物活性，粘性水凝胶作为伤口敷料已得到广泛研究。然而，大多数设计的水凝胶仍然存在局限性，包括潜在的有毒单体、复杂的制备过程和不可降解的特性。在此，通过酶交联制备了一种天然可降解的明胶/酪蛋白水凝胶。该水凝胶可以在壳聚糖（CS）溶液的介导下按需粘附于组织。研究发现，水凝胶的粘附强度可以通过调节明胶/酪蛋白比例、EDC&NHS浓度、CS浓度、甘油含量和交联度来控制。为了进一步扩大水凝胶的适用性，可以通过改变转谷氨酰胺酶（TG）浓度来调节水凝胶的降解和药物释放速率。此外，将盐酸四环素（TH）作为药物模型负载到水凝胶中，赋予水凝胶良好的抗菌性能。结果表明，0.03%TH水凝胶具有优异的血液相容性和细胞相容性，并且可以促进小鼠感染伤口的愈合。本研究为生物医学工程领域提供了一种新型天然粘性水凝胶。
