Barat M, Rickwood D, Dufresne C, Mounolou J C
Exp Cell Res. 1985 Mar;157(1):207-17. doi: 10.1016/0014-4827(85)90163-6.
Mitochondrial DNA (mtDNA)-protein complexes (nucleoids) from Xenopus laevis oocytes were purified either on rate-zonal sucrose or isopyknic metrizamide gradients. From electron microscopic studies and staphylococcal nuclease digestion experiments mtDNA appears to be packaged into regular beaded structures. Protein electrophoretic analysis and M banding results show that mtDNA is associated with the membrane structures and also with few specific proteins including one acid-soluble polypeptide of 28 kD.
来自非洲爪蟾卵母细胞的线粒体DNA（mtDNA）-蛋白质复合物（类核）通过速率区带蔗糖梯度或等密度氯化铯梯度进行纯化。通过电子显微镜研究和葡萄球菌核酸酶消化实验发现，mtDNA似乎被包装成规则的珠状结构。蛋白质电泳分析和M带分析结果表明，mtDNA与膜结构相关，也与少数特定蛋白质相关，包括一种28kD的酸溶性多肽。
