对非洲爪蟾一种对超螺旋DNA具有高亲和力的线粒体蛋白的特性分析。
Characterization of a Xenopus laevis mitochondrial protein with a high affinity for supercoiled DNA.
作者信息
Mignotte B, Barat M
出版信息
Nucleic Acids Res. 1986 Aug 11;14(15):5969-80. doi: 10.1093/nar/14.15.5969.
A DNA binding protein of 31 Kd -mtDBPC- has been isolated from X. laevis oocyte mitochondria. It is present in large amounts in the organelle and does not show any enzymatic activity. Its binding to the superhelical form of a DNA is higher than for any other form, or for RNA. No sequence specificity could be found for any mtDNA fragments tested, including both origins of replication. It is able to introduce superhelical turns into relaxed circular DNA in the presence of a topoisomerase I activity. It could be a component of the mitochondrial nucleoids.
一种31千道尔顿的DNA结合蛋白——线粒体DNA结合蛋白（mtDBPC）已从非洲爪蟾卵母细胞线粒体中分离出来。它大量存在于该细胞器中，且不表现出任何酶活性。它与超螺旋形式的DNA的结合能力高于与任何其他形式的DNA或RNA的结合能力。对于所测试的任何线粒体DNA片段，包括两个复制起点，均未发现序列特异性。在拓扑异构酶I活性存在的情况下，它能够将超螺旋结构引入松弛的环状DNA中。它可能是线粒体核仁的一个组成部分。
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