Vaez Hamid, Yazdanpour Zahra
Department of Microbiology School of Medicine, Zabol University of Medical Sciences Zabol Iran.
Department of Microbiology School of Medicine, Kermanshah University of Medical Sciences Kermanshah Iran.
Health Sci Rep. 2024 Dec 24;7(12):e70309. doi: 10.1002/hsr2.70309. eCollection 2024 Dec.
(), included in the World Health Organization's list of critical priority pathogens, is considered a serious threat to public health. The present study aims to investigate the prevalence of virulence-associated and aminoglycoside resistance genes in clinical isolates of .
This cross-sectional study was carried out on 88 clinical isolates of . collected from patients at Zabol hospital, Iran. Isolates were identified using conventional microbiology tests and polymerase chain reaction (PCR). Antibiotic susceptibility patterns were ascertained by the disc diffusion method. The prevalence of virulence-associated genes (, and ) and aminoglycoside resistance (AME) genes (, , , , , and ) was investigated by PCR.
The isolates were mostly resistant to kanamycin (73.8%) and streptomycin (69.3%). The most predominant virulence gene was , observed in 89.8% of isolates, followed by 55.7% and 14.8%. The most prevalent resistance gene was , which was detected in 35.2% of isolates, followed by 22.7% and 17%. In addition, sixteen different patterns of AME genes were observed.
Most investigated isolates of were positive for different virulence-associated and AME genes and therefore can play a significant role in life-threatening infections. Meanwhile, resistance rates to aminoglycoside antibiotics were high and it was primarily due to the presence of AME genes such as , ant , and .
（某病原体，原文未明确写出）被列入世界卫生组织重点关注病原体清单，被认为是对公众健康的严重威胁。本研究旨在调查（该病原体）临床分离株中毒力相关基因和氨基糖苷类抗性基因的流行情况。
本横断面研究对88株（该病原体）临床分离株进行。这些分离株从伊朗扎博勒医院的患者中收集。采用传统微生物学检测和聚合酶链反应（PCR）对分离株进行鉴定。通过纸片扩散法确定抗生素敏感性模式。通过PCR研究毒力相关基因（具体基因，原文未明确写出）和氨基糖苷类抗性（AME）基因（具体基因，原文未明确写出）的流行情况。
分离株大多对卡那霉素（73.8%）和链霉素（69.3%）耐药。最主要的毒力基因是（具体基因，原文未明确写出），在89.8%的分离株中观察到，其次是（具体基因，原文未明确写出）55.7%和（具体基因，原文未明确写出）14.8%。最普遍的抗性基因是（具体基因，原文未明确写出），在35.2%的分离株中检测到，其次是（具体基因，原文未明确写出）22.7%和（具体基因，原文未明确写出）17%。此外，观察到16种不同的AME基因模式。
大多数被调查的（该病原体）分离株对不同的毒力相关基因和AME基因呈阳性，因此可能在危及生命的感染中起重要作用。同时，对氨基糖苷类抗生素的耐药率很高，这主要是由于存在AME基因，如（具体基因，原文未明确写出）、ant（具体基因，原文未明确写出）和（具体基因，原文未明确写出）。
