Soliman Enas A, Saad Alaa, Abd El Tawab Ashraf A, Elhofy Fatma I, Rizk Amira M, Elkhayat Manar, Kozytska Tamara, Ilyas Majdil, Bassiouny Marwa, Brangsch Hanka, Pletz Mathias W, Neubauer Heinrich, Sprague Lisa D, Wareth Gamal
Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Toukh, Moshtohor, Egypt.
Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany.
One Health. 2024 Sep 27;19:100904. doi: 10.1016/j.onehlt.2024.100904. eCollection 2024 Dec.
is a ubiquitous nosocomial pathogen associated with various types of infections in hospitalized patients and different animal species. In the current study, 49 strains isolated from humans, dogs, and cats were investigated using NGS technology. MALDI-TOF failed to identify newly discovered and isolates correctly. MLST analysis revealed different sequence types among isolates, and the most frequent STs were ST29, ST219, and ST37. Three ST23 that are generally known as hypervirulent type were identified but they lacked major discriminatory determinants for hypervirulent (hvKp). isolates showed high diversity, and several isolates from humans and animals were assigned to the same ST and were almost identical. Isolates from humans exhibited more pronounced resistance patterns compared to the animal isolates. High levels of resistance were observed for piperacillin, trimethoprim/sulfamethoxazole, and cephalosporins, and resistance to carbapenem compounds was only found in isolates of human origin. Three strains of human origin were extensively drug-resistant (XDR). A diverse range of resistance genes primarily confer resistance to beta-lactams., phenicol/quinolone, aminoglycoside, macrolide, sulfonamides, and fosfomycin were identified in silico However, there were inconsistencies between the phenotypic characterization of isolates and the set of resistance genes detected in silico in this set of isolates. Further research using a larger number of isolates from various sources is necessary to fully comprehend the relationship between the presence of antimicrobial resistance determinants and phenotypic data. It is also necessary to monitor the spread of from a One Health perspective in Egypt.
是一种常见的医院病原体，与住院患者和不同动物物种的各种感染有关。在本研究中，使用二代测序（NGS）技术对从人、狗和猫分离出的49株菌株进行了调查。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）未能正确鉴定新发现的菌株和分离株。多位点序列分型（MLST）分析揭示了分离株之间不同的序列类型，最常见的序列型为ST29、ST219和ST37。鉴定出三个通常被称为高毒力型的ST23，但它们缺乏高毒力肺炎克雷伯菌（hvKp）的主要鉴别决定因素。分离株表现出高度多样性，来自人和动物的几个分离株被归为同一序列型且几乎相同。与人源分离株相比，动物源分离株的耐药模式更为明显。观察到对哌拉西林、甲氧苄啶/磺胺甲恶唑和头孢菌素的高耐药水平，仅在人源分离株中发现对碳青霉烯类化合物的耐药性。三株人源菌株为广泛耐药（XDR）。在计算机分析中鉴定出多种耐药基因，主要赋予对β-内酰胺类、氯霉素/喹诺酮类、氨基糖苷类、大环内酯类、磺胺类和磷霉素的耐药性。然而，在这组分离株中，分离株的表型特征与计算机检测到的耐药基因集之间存在不一致。有必要使用来自各种来源的大量分离株进行进一步研究，以充分理解抗菌药物耐药性决定因素的存在与表型数据之间的关系。从“同一个健康”的角度监测埃及肺炎克雷伯菌的传播也很有必要。
