Nolla H, Bristol J R, Mayberry L F
Exp Parasitol. 1985 Apr;59(2):180-4. doi: 10.1016/0014-4894(85)90070-0.
Glucose absorption and net small intestinal water movement were examined in rats infected with Nippostrongylus brasiliensis at Days 4, 6, 9, 13, and 19 after inoculation. Rats were infected with 4 X 10(3) N. brasiliensis third stage larvae. The entire small intestine was divided into three segments and each segment perfused simultaneously in vivo with Krebs-Ringer phosphate buffer containing 80 mM glucose, 6 X 10(5) dpm/ml [3H]glucose, and 6.2 X 10(3) dpm/ml [14C]polyethylene glycol. Rats perfused on Days 6, 9, 13, and 19 after inoculation showed a significant (P less than 0.05) decrease in glucose absorption rates from all three segments of the small intestine when compared to uninfected controls. In the three segments of uninfected rat small intestine and those perfused on Days 4, 13, and 19 after inoculation, net absorption of water occurred. However, in the proximal and distal segments perfused on Day 6 and the proximal segment perfused on Day 9, net water movement into the lumen occurred. This is the first report of depressed glucose absorption along the entire length of the small intestine during nippostrongylosis and contradicts previous reports of unaltered net glucose absorption in response to this parasite.
在接种巴西日圆线虫后的第4、6、9、13和19天，对感染该线虫的大鼠的葡萄糖吸收和小肠净水分移动情况进行了检测。大鼠感染了4×10³条巴西日圆线虫第三期幼虫。将整个小肠分为三段，并在体同时用含有80 mM葡萄糖、6×10⁵ dpm/ml [³H]葡萄糖和6.2×10³ dpm/ml [¹⁴C]聚乙二醇的 Krebs-Ringer 磷酸盐缓冲液灌注每一段。与未感染的对照组相比，在接种后第6、9、13和19天进行灌注的大鼠，其小肠所有三段的葡萄糖吸收率均显著降低（P<0.05）。在未感染大鼠的小肠三段以及接种后第4、13和19天进行灌注的小肠段中，发生了水的净吸收。然而，在第6天灌注的近端和远端小肠段以及第9天灌注的近端小肠段中，水向肠腔的净移动发生了。这是关于在感染巴西日圆线虫期间小肠全长葡萄糖吸收降低的首次报道，与之前关于该寄生虫感染后葡萄糖净吸收未改变的报道相矛盾。
