Li Baokun, Wang Junjie, Wang Xingjian, Jiang Biaobiao, Niu Qingfen
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China; Natural Products Research Center of Guizhou Province, Guiyang 550014, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Mar 15;329:125585. doi: 10.1016/j.saa.2024.125585. Epub 2024 Dec 10.
Considering the high toxicity of Hg to living organisms, accurately and sensitively detecting Hg in environments, biology and food systems is of the utmost importance. Herein, we present a new fast-responsive oligothiophene-derivatized fluorescent sensor TTB for Hg detection in real water/soil/urine/food samples. Sensor TTB shows a quick response, ultrahigh specificity, strong anti-disturbance, high-sensitivity with a satisfactory detection limit of 0.23 µM and good reversibility towards Hg. The response mechanism was well examined through Job's plot, FTIR spectra and DFT calculations. To examine naked-eye recognition of Hg, a visualization method by the TTB-coated test paper and cotton swabs was used for quickly detecting Hg. Additionally, sensor TTB shows good application flexibility and proves the capability for quantitative and accurate monitoring of Hg levels in various water, soil, human urine, tea and seafood samples with an excellent efficiency. Moreover, fluorescence imaging experiments demonstrated that the TTB showed good cell-permeability and was utilized to detect/image Hg in living plants. This study provides a powerful tool for accurately and reliably detecting Hg in bio/environmental and food systems.
鉴于汞对生物的高毒性，在环境、生物和食品系统中准确、灵敏地检测汞至关重要。在此，我们展示了一种新型的快速响应的寡噻吩衍生荧光传感器TTB，用于在实际水/土壤/尿液/食品样品中检测汞。传感器TTB显示出快速响应、超高特异性、强抗干扰性、高灵敏度，检测限令人满意，为0.23 μM，并且对汞具有良好的可逆性。通过Job曲线、傅里叶变换红外光谱（FTIR）和密度泛函理论（DFT）计算对响应机制进行了深入研究。为了检验对汞的肉眼识别，采用了用TTB包被的试纸和棉签的可视化方法来快速检测汞。此外，传感器TTB显示出良好的应用灵活性，并证明能够高效地定量和准确监测各种水、土壤、人类尿液、茶叶和海鲜样品中的汞含量。此外，荧光成像实验表明，TTB具有良好的细胞渗透性，可用于检测/成像活植物中的汞。本研究为在生物/环境和食品系统中准确可靠地检测汞提供了一个有力工具。
