Wang Sipei, Jin Shanshan, Zhu Xiangjin, Li Yuan, Pan Xinling
Department of Clinical Laboratory, Wenzhou Medical University Affiliated Dongyang Hospital, Dongyang, Zhejiang, People's Republic of China.
Department of Biomedical Sciences Laboratory, Wenzhou Medical University Affiliated Dongyang Hospital, Dongyang, Zhejiang, People's Republic of China.
Infect Drug Resist. 2024 Dec 19;17:5629-5637. doi: 10.2147/IDR.S500969. eCollection 2024.
Diarrhea caused by non-O1/O139-group e (NOVC) tends to be mild and can be readily overlooked. In this report, a NOVC strain designated XXM was isolated from the blood of a 68-year-old male undergoing surgical treatment for a bile duct malignancy in October 2023.
XXM was identified through a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Virulence genes were detected using a virulence gene dual real-time fluorescent PCR kit. AST-GN13 and AST-GN334 cards were used to test the resistance against 16 antibiotics with a Vitek2 compact system. The genomic and phylogenetic characteristics of XXM were established through whole genome sequencing (WGS).
Serum agglutination tests revealed the isolate to be a non-O1/non-O139 strain. The strain was sensitive to all 16 tested antibiotics and did not carry the gene. MLST analyses identified the XXM strain as ST1538. WGS analyses identified 8 classes of virulence genes with different functions. A total of 3.541 bacterial genes, including 3.482 from , were annoted using the Non-Redundant Protein Sequence (NR) database. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses annotated 32 genes including 17 key proteins involved in the biofilm pathway. Comparative analyses using the Pathogen Host Interactions Database (PHI) identified the gene. Evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) was used to annotate 3280 genes in 21 categories. Phylogenetic analyses revealed that strain XXM was closely related to strain Man9.
The XXM carries multiple virulence genes, and this genomic analysis of the XXM in comparison with other NOVC strains provides important information for an improved understanding of the pathogenicity of NOVC in clinical samples.
非O1/O139群霍乱弧菌（NOVC）引起的腹泻往往较为轻微，容易被忽视。在本报告中，2023年10月从一名因胆管恶性肿瘤接受手术治疗的68岁男性血液中分离出一株名为XXM的NOVC菌株。
通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）鉴定XXM。使用毒力基因双重实时荧光PCR试剂盒检测毒力基因。使用AST-GN13和AST-GN334卡片，通过Vitek2 compact系统检测对16种抗生素的耐药性。通过全基因组测序（WGS）确定XXM的基因组和系统发育特征。
血清凝集试验显示该分离株为非O1/非O139菌株。该菌株对所有16种测试抗生素敏感，且未携带相关基因。多位点序列分型（MLST）分析将XXM菌株鉴定为ST1538。WGS分析鉴定出8类具有不同功能的毒力基因。使用非冗余蛋白质序列（NR）数据库注释了总共3541个细菌基因，其中包括来自的3482个基因。京都基因与基因组百科全书（KEGG）分析注释了32个基因，其中包括17个参与生物膜途径的关键蛋白。使用病原体-宿主相互作用数据库（PHI）进行的比较分析鉴定出相关基因。基因进化谱系：非监督直系同源组（eggNOG）用于注释21类中的3280个基因。系统发育分析表明，XXM菌株与霍乱弧菌菌株Man9密切相关。
XXM携带多个毒力基因，与其他NOVC菌株相比，对XXM的这种基因组分析为更好地了解临床样本中NOVC的致病性提供了重要信息。
