Tang Jinrong, Li Sheng, Zhang Manli, Li Fengzhi, Tang Ying, Yang Fangfang
Department of Clinical Laboratory, Mianyang Hospital of Traditional Chinese Medicine/Mianyang Hospital Affiliated to Chengdu University of Traditional Chinese Medicine, Mianyang, Sichuan, 621000, People's Republic of China.
Youxian District Center for Disease Control and Prevention of Mianyang City, Mianyang, Sichuan, 621000, People's Republic of China.
Infect Drug Resist. 2023 Aug 21;16:5453-5461. doi: 10.2147/IDR.S420095. eCollection 2023.
Non-O1, non-O139 (NOVC) can cause cholera-like diarrhea, but it rarely causes extraintestinal infection, so it is easily overlooked. In this report, we present a case of NOVC detected through blood culture in a 58-year-old male patient with cirrhosis, resulting in severe infection. The patient had been diagnosed with cirrhosis seven years prior and was admitted to the hospital due to abdominal distension and gastrointestinal bleeding. Gram-negative bacilli were isolated from blood cultures and identified as using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and average nucleotide identity (ANI). Moreover, the serum agglutination test showed that the strain was non-O1/non-O139. Further whole genome sequencing and analysis of the strain showed that the strain mainly carried virulence genes , but no resistant genes such as were detected. It provides information for the study of the pathogenic mechanism and drug resistance mechanism of . The patient had severe symptoms and a poor prognosis, indicating that although the NOVC strain infected in this patient had few virulence genes, it was not weak in pathogenicity. It may be caused by the effect of some virulence genes, which should be paid attention to.
非O1、非O139（NOVC）可引起霍乱样腹泻，但很少引起肠外感染，因此很容易被忽视。在本报告中，我们介绍了一例通过血培养在一名58岁肝硬化男性患者中检测到NOVC的病例，该病例导致了严重感染。该患者七年前被诊断为肝硬化，因腹胀和胃肠道出血入院。从血培养中分离出革兰氏阴性杆菌，并使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）和平均核苷酸同一性（ANI）进行鉴定。此外，血清凝集试验表明该菌株为非O1/非O139。对该菌株进一步进行全基因组测序和分析表明，该菌株主要携带毒力基因，但未检测到如 等耐药基因。它为研究 的致病机制和耐药机制提供了信息。该患者症状严重，预后不良，这表明尽管该患者感染的NOVC菌株毒力基因较少，但其致病性并不弱。这可能是由某些毒力基因的作用引起的，应予以关注。
