Department of Clinical Laboratory, Sanmen People's Hospital, Taizhou, Zhejiang, China.
Department of Clinical Laboratory, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Taizhou, Zhejiang, China.
Microbiol Spectr. 2023 Aug 17;11(4):e0205423. doi: 10.1128/spectrum.02054-23. Epub 2023 Jun 28.
The increasing incidence of non-O1/non-O139 Vibrio cholerae (NOVC) has been observed worldwide. However, septicemia caused by NOVC remains a rare condition that has received limited attention. Currently, there are no established treatment guidelines for bloodstream infections caused by NOVC, and the understanding of this condition mainly relies on individual case reports. Although NOVC bacteremia can be fatal in a small percentage of cases, knowledge about its microbiological features remains limited. Here, we present a case of V. cholerae septicemia caused by NOVC in a 46-year-old man with chronic viral hepatitis and liver cirrhosis. The isolated strain, named V. cholerae VCH20210731 and classified as a new sequence type (ST), ST1553, was found to be susceptible to most of the antimicrobial agents tested. O-antigen serotyping of V. cholerae VCH20210731 revealed that it belonged to serotype Ob5. Interestingly, the genes, which are typically associated with V. cholerae, were absent in VCH20210731. However, the strain possessed 25 other potential virulence genes, such as , , , and . The resistome of V. cholerae VCH20210731 included several genes, including , , , and . Nevertheless, susceptibility testing demonstrated that the isolate was susceptible to most of the antimicrobial agents tested. Phylogenetic analysis indicated that the closest strain to VCH20210731 was strain 120 from Russia, differing by 630 single-nucleotide polymorphisms (SNPs). Our findings contribute to the understanding of the genomic epidemiological characteristics and antibiotic resistance mechanisms of this invasive bacterial pathogen. This study highlights the discovery of a novel ST1553 V. cholerae strain in China, providing valuable insights into the genomic epidemiology and global transmission dynamics of V. cholerae. It is important to note that clinical presentations of NOVC bacteremia can vary significantly, and the isolates demonstrate genetic diversity. Consequently, health care professionals and public health experts should remain vigilant about the potential for infection with this pathogen, particularly considering the elevated prevalence of liver disease in China.
全球范围内观察到非 O1/O139 霍乱弧菌（NOVC）的发病率不断上升。然而，NOVC 引起的败血症仍然是一种罕见的病症，受到的关注有限。目前，尚无针对 NOVC 引起的血流感染的既定治疗指南，对该病的了解主要依赖于个别病例报告。尽管 NOVC 菌血症在极少数情况下可能致命，但对其微生物学特征的了解仍然有限。在这里，我们报告了一例由 NOVC 引起的 46 岁慢性病毒性肝炎和肝硬化男性霍乱弧菌败血症。分离株命名为霍乱弧菌 VCH20210731，被归类为一个新的序列型（ST），ST1553，对大多数测试的抗菌药物均敏感。霍乱弧菌 VCH20210731 的 O 抗原血清型鉴定表明其属于血清型 Ob5。有趣的是，霍乱弧菌通常与之相关的 基因在 VCH20210731 中缺失。然而，该菌株拥有 25 个其他潜在的毒力基因，如 、 、 、 。霍乱弧菌 VCH20210731 的耐药组包括几个基因，如 、 、 、 。然而，药敏试验表明该分离株对大多数测试的抗菌药物均敏感。系统发育分析表明，与 VCH20210731 最接近的菌株是来自俄罗斯的菌株 120，相差 630 个单核苷酸多态性（SNP）。我们的研究结果有助于了解这种侵袭性细菌病原体的基因组流行病学特征和抗生素耐药机制。本研究强调了在中国发现一种新型 ST1553 霍乱弧菌菌株，为霍乱弧菌的基因组流行病学和全球传播动态提供了有价值的见解。值得注意的是，NOVC 菌血症的临床表现可能有很大差异，并且分离株表现出遗传多样性。因此，临床医生和公共卫生专家应该对感染这种病原体的潜在风险保持警惕，特别是考虑到中国肝病的高发率。
