Daza Prieto Beatriz, Raicevic Nadja, Martinovic Aleksandra, Ladstätter Johann, Zuber Bogdanovic Ivana, Schorpp Anika, Stoeger Anna, Mach Robert L, Ruppitsch Werner, Cabal Adriana
Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Vienna, Austria.
Centre of Excellence for Digitalisation of Microbial Food Safety Risk Assessment and Quality Parameters for Accurate Food Authenticity Certification, University of Dona Gorica, Podgorica, Montenegro.
Front Microbiol. 2024 Dec 11;15:1473938. doi: 10.3389/fmicb.2024.1473938. eCollection 2024.
is a widespread acid-lactic bacterium found in the environment, humans, and animal microbiota, and it also plays a role in the production of traditional food. However, the worldwide emergence of multidrug-resistant strains represents a major public health threat and is the primary reason that the genus is not recommended for the Qualified Presumption of Safety (QPS) list of the European Food Safety Authority (EFSA), raising concerns about its presence in food products.
In this study, 39 and 5 isolates were obtained from artisanal brine cheeses and dry sausages, sourced from 21 different Montenegrin producers. The isolates were collected following the ISO 15214:1998 international method and processed for whole-genome sequencing (WGS).
Genome analysis based on core genome multilocus sequence type (cgMLST) revealed a high diversity among isolates. Furthermore, the isolates carried antimicrobial resistance genes; the virulence genes , , and ; the bacteriocin genes , , and ; the secondary metabolite genes , cyclic lactone autoinducer, RiPP-like, and and a maximum of eight plasmids.
This study highlights the need for careful monitoring of and strains in food to ensure they do not pose any potential risks to consumer safety.
乳酸杆菌是一种广泛存在于环境、人类和动物微生物群中的细菌，它在传统食品生产中也发挥着作用。然而，全球范围内多重耐药菌株的出现构成了重大的公共卫生威胁，这也是欧洲食品安全局（EFSA）的合格安全推定（QPS）名单中不推荐该属细菌的主要原因，引发了人们对其在食品中存在的担忧。
在本研究中，从来自21个不同黑山生产商的手工盐水奶酪和干香肠中获得了39株乳酸杆菌和5株分离株。按照ISO 15214:1998国际方法收集分离株，并进行全基因组测序（WGS）。
基于核心基因组多位点序列分型（cgMLST）的基因组分析显示分离株之间具有高度多样性。此外，分离株携带抗微生物抗性基因；毒力基因、和；细菌素基因、和；次生代谢物基因、环状内酯自诱导物、类核糖体合成和翻译后修饰肽（RiPP）样基因以及和最多八个质粒。
本研究强调需要仔细监测食品中的乳酸杆菌和分离株，以确保它们不会对消费者安全构成任何潜在风险。
